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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 2 — Jan. 19, 2009
  • pp: 784–796

Three-dimensional endomicroscopy of the human colon using optical coherence tomography

Desmond C. Adler, Chao Zhou, Tsung-Han Tsai, Joe Schmitt, Qin Huang, Hiroshi Mashimo, and James G. Fujimoto  »View Author Affiliations

Optics Express, Vol. 17, Issue 2, pp. 784-796 (2009)

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Three-dimensional (3D) endomicroscopy imaging of the human gastrointestinal tract is demonstrated in vivo using a swept source optical coherence tomography (OCT) system. 3D datasets of normal and pathologic regions of the colon, rectum, and anal verge were obtained from seven volunteers undergoing diagnostic or therapeutic colonoscopy. 3D-OCT enables high resolution endomicroscopy examination through visualization of tissue architectural morphology using virtual cross-sectional images with arbitrary orientations as well as en face projection images. Axial image resolutions of 6 μm in tissue are obtained over a ~180 mm2 field with an imaging range of 1.6 mm. A Fourier domain mode locked (FDML) laser providing a tuning range of 180 nm at a sweep rate of 62 kHz is used as the system light source. This clinical pilot study demonstrates the potential of 3D-OCT for distinguishing normal from pathologic colorectal tissue, assessing endoscopic therapies and healing progression.

© 2009 Optical Society of America

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2680) Medical optics and biotechnology : Gastrointestinal
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: November 7, 2008
Revised Manuscript: December 30, 2008
Manuscript Accepted: January 3, 2009
Published: January 8, 2009

Virtual Issues
Vol. 4, Iss. 3 Virtual Journal for Biomedical Optics

Desmond C. Adler, Chao Zhou, Tsung-Han Tsai, Joe Schmitt, Qin Huang, Hiroshi Mashimo, and James G. Fujimoto, "Three-dimensional endomicroscopy of the human colon using optical coherence tomography," Opt. Express 17, 784-796 (2009)

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