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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 22 — Oct. 26, 2009
  • pp: 19382–19400

Optics InfoBase > Optics Express > Volume 17 > Issue 22 > Page 19382

Adaptive optics optical coherence tomography at 120,000 depth scans/s for non-invasive cellular phenotyping of the living human retina

Cristiano Torti, Boris Považay, Bernd Hofer, Angelika Unterhuber, Joseph Carroll, Peter Kurt Ahnelt, and Wolfgang Drexler  »View Author Affiliations


Optics Express, Vol. 17, Issue 22, pp. 19382-19400 (2009)
http://dx.doi.org/10.1364/OE.17.019382


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Abstract

This paper presents a successful combination of ultra-high speed (120,000 depth scans/s), ultra-high resolution optical coherence tomography with adaptive optics and an achromatizing lens for compensation of monochromatic and longitudinal chromatic ocular aberrations, respectively, allowing for non-invasive volumetric imaging in normal and pathologic human retinas at cellular resolution. The capability of this imaging system is demonstrated here through preliminary studies by probing cellular intraretinal structures that have not been accessible so far with in vivo, non-invasive, label-free imaging techniques, including pigment epithelial cells, micro-vasculature of the choriocapillaris, single nerve fibre bundles and collagenous plates of the lamina cribrosa in the optic nerve head. In addition, the volumetric extent of cone loss in two colour-blinds could be quantified for the first time. This novel technique provides opportunities to enhance the understanding of retinal pathogenesis and early diagnosis of retinal diseases.

© 2009 OSA

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4470) Medical optics and biotechnology : Ophthalmology
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(220.1000) Optical design and fabrication : Aberration compensation
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: August 24, 2009
Revised Manuscript: September 26, 2009
Manuscript Accepted: September 28, 2009
Published: October 12, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Cristiano Torti, Boris Považay, Bernd Hofer, Angelika Unterhuber, Joseph Carroll, Peter Kurt Ahnelt, and Wolfgang Drexler, "Adaptive optics optical coherence tomography at 120,000 depth scans/s for non-invasive cellular phenotyping of the living human retina," Opt. Express 17, 19382-19400 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-22-19382


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