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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 22 — Oct. 26, 2009
  • pp: 19674–19681

Assessing light scattering of intracellular organelles in single intact living cells

Maxim Kalashnikov, Wonshik Choi, Chung-Chieh Yu, Yongjin Sung, Ramachandra R. Dasari, Kamran Badizadegan, and Michael S. Feld  »View Author Affiliations


Optics Express, Vol. 17, Issue 22, pp. 19674-19681 (2009)
http://dx.doi.org/10.1364/OE.17.019674


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Abstract

This report presents a model-independent method of assessing contributions to the light scattering from individual organelles in single intact cells. We first measure the 3D index map of a living cell, and then modify the map in such a way so as to eliminate contrast due to a particular intracellular organelle. By calculating and comparing the light scattering distributions calculated from the original and modified index maps using the Rytov approximation, we extract the light scattering contribution from the particular organelle of interest. The relative contributions of the nucleus and nucleolus to the scattering of the entire cell are thus determined, and the applicability of the homogeneous spherical model to non-spherical and heterogeneous organelles in forward scattering is evaluated.

© 2009 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(290.4020) Scattering : Mie theory
(290.5820) Scattering : Scattering measurements
(290.2558) Scattering : Forward scattering

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: August 13, 2009
Revised Manuscript: September 27, 2009
Manuscript Accepted: September 28, 2009
Published: October 15, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Maxim Kalashnikov, Wonshik Choi, Chung-Chieh Yu, Yongjin Sung, Ramachandra R. Dasari, Kamran Badizadegan, and Michael S. Feld, "Assessing light scattering of intracellular organelles in single intact living cells," Opt. Express 17, 19674-19681 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-22-19674


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References

  1. V. Backman, R. Gurjar, K. Badizadegan, L. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, “Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ,” IEEE J. Sel. Top. Quantum Electron. 5(4), 1019–1026 (1999). [CrossRef]
  2. C. Mujat, C. Greiner, A. Baldwin, J. M. Levitt, F. Tian, L. A. Stucenski, M. Hunter, Y. L. Kim, V. Backman, M. Feld, K. Münger, and I. Georgakoudi, “Endogenous optical biomarkers of normal and human papillomavirus immortalized epithelial cells,” Int. J. Cancer 122(2), 363–371 (2008). [CrossRef]
  3. H. Fang, M. Ollero, E. Vitkin, L. M. Kimerer, P. B. Cipolloni, M. M. Zaman, S. D. Freedman, I. J. Bigio, I. Itzkan, E. B. Hanlon, and L. T. Perelman, “Noninvasive sizing of subcellular organelles with light scattering spectroscopy,” IEEE J. Sel. Top. Quantum Electron. 9(2), 267–276 (2003). [CrossRef]
  4. R. S. Gurjar, V. Backman, L. T. Perelman, I. Georgakoudi, K. Badizadegan, I. Itzkan, R. R. Dasari, and M. S. Feld, “Imaging human epithelial properties with polarized light-scattering spectroscopy,” Nat. Med. 7(11), 1245–1248 (2001). [CrossRef] [PubMed]
  5. M. Hunter, V. Backman, G. Popescu, M. Kalashnikov, C. W. Boone, A. Wax, V. Gopal, K. Badizadegan, G. D. Stoner, and M. S. Feld, “Tissue self-affinity and polarized light scattering in the born approximation: A new model for precancer detection,” Phys. Rev. Lett. 97(13), 138102 (2006). [CrossRef] [PubMed]
  6. J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. P. Freyer, “Polarized angular dependent spectroscopy of epithelial cells and epithelial cell nuclei to determine the size scale of scattering structures,” J. Biomed. Opt. 7(3), 378–387 (2002). [CrossRef] [PubMed]
  7. A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, “Cellular Organization and Substructure Measured Using Angle-Resolved Low-Coherence Interferometry,” Biophys. J. 82(4), 2256–2264 (2002). [CrossRef] [PubMed]
  8. A. Wax, C. Yang, M. G. Müller, R. Nines, C. W. Boone, V. E. Steele, G. D. Stoner, R. R. Dasari, and M. S. Feld, “In Situ Detection of Neoplastic Transformation and Chemopreventive Effects in Rat Esophagus Epithelium Using Angle-resolved Low-coherence Interferometry,” Cancer Res. 63(13), 3556–3559 (2003). [PubMed]
  9. J. D. Wilson and T. H. Foster, “Mie theory interpretations of light scattering from intact cells,” Opt. Lett. 30(18), 2442–2444 (2005). [CrossRef] [PubMed]
  10. M. Xu, T. T. Wu, and J. A. Y. Qu, “Unified Mie and fractal scattering by cells and experimental study on application in optical characterization of cellular and subcellular structures,” J. Biomed. Opt. 13, (2008). [PubMed]
  11. C. C. Yu, C. Lau, J. W. Tunnell, M. Hunter, M. Kalashnikov, C. Fang-Yen, S. F. Fulghum, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy,” Opt. Lett. 31(21), 3119–3121 (2006). [CrossRef] [PubMed]
  12. A. Brunsting and P. F. Mullaney, “Differential Light Scattering from Spherical Mammalian Cells,” Biophys. J. 14(6), 439–453 (1974). [CrossRef] [PubMed]
  13. R. M. P. Doornbos, M. Schaeffer, A. G. Hoekstra, P. M. A. Sloot, B. G. deGrooth, and J. Greve, “Elastic light-scattering measurements of single biological cells in an optical trap,” Appl. Opt. 35(4), 729–734 (1996). [CrossRef] [PubMed]
  14. V. P. Maltsev, “Scanning flow cytometry for individual particle analysis,” Rev. Sci. Instrum. 71(1), 243–255 (2000). [CrossRef]
  15. V. P. Maltsev, and K. A. Semyanov, Characterisation of Bio-Particles from Light Scattering (VSP, Utrecht, 2004).
  16. A. E. Zharinov, P. A. Tarasov, A. N. Shvalov, K. A. Semyanov, D. R. van Bockstaele, and V. P. Maltsev, “A study of light scattering of mononuclear blood cells with scanning flow cytometry,” J. Quant. Spectrosc. Radiat. Transf. 102(1), 121–128 (2006). [CrossRef]
  17. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4(9), 717–719 (2007). [CrossRef] [PubMed]
  18. W. Choi, C. C. Yu, C. Fang-Yen, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Field-based angle-resolved light-scattering study of single live cells,” Opt. Lett. 33(14), 1596–1598 (2008). [CrossRef] [PubMed]
  19. Y. Sung, W. Choi, C. Fang-Yen, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Optical diffraction tomography for high resolution live cell imaging,” Opt. Express 17(1), 266–277 (2009). [CrossRef] [PubMed]
  20. A. J. Devaney, “Inverse-scattering theory within the Rytov approximation,” Opt. Lett. 6(8), 374–376 (1981). [CrossRef] [PubMed]
  21. E. Wolf, “Three-dimensional structure determination of semi-transparent objects from holographic data,” Opt. Commun. 1(4), 153–156 (1969). [CrossRef]
  22. H. Hulst, Light scattering by small particles (Dover Publications, New York, 1981).
  23. C. L. Curl, C. J. Bellair, T. Harris, B. E. Allman, P. J. Harris, A. G. Stewart, A. Roberts, K. A. Nugent, and L. M. D. Delbridge, “Refractive index measurement in viable cells using quantitative phase-amplitude microscopy and confocal microscopy,” Cytometry A 65A(1), 88–92 (2005). [CrossRef]
  24. K. F. A. Ross, Phase contrast and interference microscopy for cell biologists (Edward Arnold Ltd, London, 1967).
  25. R. Barer, K. F. A. Ross, and S. Tkaczyk, “Refractometry of living cells,” Nature 171(4356), 720–724 (1953). [CrossRef] [PubMed]

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