OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 23 — Nov. 9, 2009
  • pp: 20700–20706

All-fiber CARS microscopy of live cells

Adrian F. Pegoraro, Andrew Ridsdale, Douglas J. Moffatt, John Paul Pezacki, Brian K. Thomas, Libin Fu, Liang Dong, Martin E. Fermann, and Albert Stolow  »View Author Affiliations


Optics Express, Vol. 17, Issue 23, pp. 20700-20706 (2009)
http://dx.doi.org/10.1364/OE.17.020700


View Full Text Article

Enhanced HTML    Acrobat PDF (1012 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Using an all-fiber laser system consisting of a femtosecond Er/Yb fiber oscillator as the pump and an ultra-highly nonlinear fiber for Stokes generation, we demonstrate multimodal (TPF+SHG+CARS) nonlinear optical microscopy of both tissue samples and live cells. Multimodal imaging was successfully performed with pixel dwell times as short as 4 µs at low laser powers (<40 mW total).

© 2009 Optical Society of America

OCIS Codes
(170.5810) Medical optics and biotechnology : Scanning microscopy
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: September 16, 2009
Revised Manuscript: October 19, 2009
Manuscript Accepted: October 20, 2009
Published: October 27, 2009

Virtual Issues
Vol. 4, Iss. 13 Virtual Journal for Biomedical Optics

Citation
Adrian F. Pegoraro, Andrew Ridsdale, Douglas J. Moffatt, John P. Pezacki, Brian K. Thomas, Libin Fu, Liang Dong, Martin E. Fermann, and Albert Stolow, "All-fiber CARS microscopy of live cells," Opt. Express 17, 20700-20706 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-23-20700


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. J.-X. Cheng and X. Xie, "Coherent anti-stokes raman scattering microscopy: Instrumentation, theory, and applications," J. Phys. Chem. B 108, 827-840 (2004). [CrossRef]
  2. E. O. Potma and X. S. Xie, "Cars microscopy for biology and medicine," Opt. Photon. News 15, 40-45 (2004). [CrossRef]
  3. D. J. Jones, E. O. Potma, J.-X. Cheng, B. Burfeindt, Y. Pang, J. Ye, and X. S. Xie, "Synchronization of two passively mode-locked, picosecond lasers within 20 fs for coherent anti-stokes raman scattering microscopy," Review of Scientific Instruments 73, 2843-2848 (2002). [CrossRef]
  4. F. Ganikhanov, S. Carrasco, X. S. Xie, M. Katz, W. Seitz, and D. Kopf, "Broadly tunable dual-wavelength light source for coherent anti-stokes raman scattering microscopy," Opt. Lett. 31, 1292-1294 (2006). [CrossRef] [PubMed]
  5. V. Yakovlev and G. I. Petrov, "Enhancing red-shifted white-light continuum generation in optical fibers for applications in nonlinear raman microscopy," Opt. Express 13, 1299-1306 (2005). [CrossRef] [PubMed]
  6. H. N. Paulsen, K. M. Hilligsøe, J. Thøgersen, S. R. Keiding, and J. J. Larsen, "Coherent anti-stokes raman scattering microscopy with a photonic crystal fiber based light source," Opt. Lett. 28, 1123-1125 (2003). [CrossRef] [PubMed]
  7. E. R. Andresen, H. N. Paulsen, V. Birkedal, J. Thøgersen, and S. R. Keiding, "Broadband multiplex coherent anti-stokes raman scattering microscopy employing photonic-crystal fibers," J. Opt. Soc. Am. B 22, 1934-1938 (2005). [CrossRef]
  8. A. F. Pegoraro, A. Ridsdale, D. J. Moffatt, Y. Jia, J. P. Pezacki, and A. Stolow, "Optimally chirped multimodal cars microscopy based on a single ti:sapphireoscillator," Opt. Express 17, 2984-2996 (2009). [CrossRef] [PubMed]
  9. W. Denk, J. Strickler, and W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73-76 (1990). [CrossRef] [PubMed]
  10. P. J. Campagnola, M.-d. Wei, A. Lewis, and L. M. Loew, "High-Resolution Nonlinear Optical Imaging of Live Cells by Second Harmonic Generation," Biophys. J. 77, 3341-3349 (1999). [CrossRef] [PubMed]
  11. K. Kieu, B. G. Saar, G. R. Holtom, X. S. Xie, and F. W. Wise, "High-power picosecond fiber source for coherent raman microscopy," Opt. Lett. 34, 2051-2053 (2009). [CrossRef] [PubMed]
  12. E. R. Andresen, C. K. Nielsen, J. Thøgersen, and S. R. Keiding, "Fiber laser-based light source for coherent anti-stokes raman scattering microspectroscopy," Opt. Express 15, 4848-4856 (2007). [CrossRef] [PubMed]
  13. G. Krauss, T. Hanke, A. Sell, D. Tr¨autlein, A. Leitenstorfer, R. Selm, M. Winterhalder, and A. Zumbusch, "Compact coherent anti-stokes raman scattering microscope based on a picosecond two-color er:fiber laser system," Opt. Lett. 34, 2847-2849 (2009). [CrossRef] [PubMed]
  14. F. L’egar’e, C. L. Evans, F. Ganikhanov, and X. S. Xie, "Towards cars endoscopy," Opt. Express 14, 4427-4432 (2006). [CrossRef] [PubMed]
  15. K. M. Hilligsøe, T. Andersen, H. Paulsen, C. Nielsen, K. Mølmer, S. Keiding, R. Kristiansen, K. Hansen, and J. Larsen, "Supercontinuum generation in a photonic crystal fiber with two zero dispersion wavelengths," Opt. Express 12, 1045-1054 (2004). [CrossRef] [PubMed]
  16. L. Fu, B. K. Thomas, and L. Dong, "Efficient supercontinuum generations in silica suspended core fibers," Opt. Express 16, 19629-19642 (2008). [CrossRef] [PubMed]
  17. K. Tada and N. Karasawa, "Broadband coherent anti-stokes raman scattering spectroscopy using soliton pulse trains from a photonic crystal fiber," Optics Communications 282, 3948 - 3952 (2009). [CrossRef]
  18. A. Efimov and A. J. Taylor, "Supercontinuum generation and soliton timing jitter in sf6 soft glass photonic crystal fibers," Opt. Express 16, 5942-5953 (2008). [CrossRef] [PubMed]
  19. K. L. Corwin, N. R. Newbury, J. M. Dudley, S. Coen, S. A. Diddams, B. R. Washburn, K. Weber, and R. S. Windeller, "Fundamental amplitude noise limitations to supercontinuum spectra generated in a microstructured fiber," App. Phys. B 77, 269-277 (2003). [CrossRef]
  20. B. Washburn and N. Newbury, "Phase, timing, and amplitude noise on supercontinua generated in microstructure fiber," Opt. Express 12, 2166-2175 (2004). [CrossRef] [PubMed]
  21. J. M. Dudley, G. Genty, and S. Coen, "Supercontinuum generation in photonic crystal fiber," Reviews of Modern Physics 78, 1135 (2006). [CrossRef]
  22. M. Shiomi, T. Ito, S. Yamada, S. Kawashima, and J. Fan, "Development of an Animal Model for Spontaneous Myocardial Infarction (WHHLMI Rabbit)," Arterioscler Thromb Vasc Biol 23, 1239-1244 (2003). [CrossRef] [PubMed]
  23. E. T. J. Nibbering, D. A. Wiersma, and K. Duppen, "Ultrafast nonlinear spectroscopy with chirped optical pulses," Phys. Rev. Lett. 68, 514-517 (1992). [CrossRef] [PubMed]
  24. T. Hellerer, A. M. Enejder, and A. Zumbusch, "Spectral focusing: High spectral resolution spectroscopy with broad-bandwidth laser pulses," Appl. Phys. Lett. 85, 25-27 (2004). [CrossRef]
  25. Ultrafast Lasers: Technology and Applications (Marcel Dekker Inc., 2003).
  26. A. M. Zheltikov, "Let there be white light: supercontinuum generation by ultrashort laser pulses," Physics-Uspekhi 49, 605-628 (2006). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 
Fig. 4.
 

Supplementary Material


» Media 1: AVI (3727 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited