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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 25 — Dec. 7, 2009
  • pp: 22281–22295

Three-color multiplex CARS for fast imaging and microspectroscopy in the entire CH n stretching vibrational region

Jae Yong Lee, Se-Hwa Kim, Dae Won Moon, and Eun Seong Lee  »View Author Affiliations

Optics Express, Vol. 17, Issue 25, pp. 22281-22295 (2009)

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We present a three-color multiplex coherent anti-Stokes Raman scattering (CARS) setup that facilitates a prompt recording of broadband CARS spectra along with a fast CARS imaging. With separate narrowband Stokes and probe beams being introduced in the near IR, we are able to incorporate a stable, wideband Ti:sapphire femtosecond laser as a pump beam that covers the full range of Raman shift for CHn stretching vibrational modes. Experimentally, high-resolution multiplex CARS signals are allowed to investigate molecular vibrations over the range of 2650 cm-1–3050 cm-1, which are spectrally integrated to construct lipid-sensitive images. It is demonstrated that the proposed implementation promises a particular benefit on CARS imaging of lipid-rich tissue structures by providing detailed information on CHn Raman-active vibrations at points of interest on the CARS images that can be obtained at high frame rates.

© 2009 Optical Society of America

OCIS Codes
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: October 7, 2009
Revised Manuscript: November 10, 2009
Manuscript Accepted: November 20, 2009
Published: November 23, 2009

Virtual Issues
Vol. 5, Iss. 1 Virtual Journal for Biomedical Optics

Jae Yong Lee, Se-Hwa Kim, Dae Won Moon, and Eun Seong Lee, "Three-color multiplex CARS for fast imaging and microspectroscopy in the entire CHn stretching vibrational region," Opt. Express 17, 22281-22295 (2009)

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