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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 25 — Dec. 7, 2009
  • pp: 22879–22889

Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry

Charles H. Camp Jr., Siva Yegnanarayanan, Ali A. Eftekhar, Hamsa Sridhar, and Ali Adibi  »View Author Affiliations

Optics Express, Vol. 17, Issue 25, pp. 22879-22889 (2009)

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Flow cytometry is an ever-advancing high-throughput multivariate analysis tool that natively provides size and morphological information. To obtain molecular information, however, typically requires the addition of fluorophores, which are limited by spectral overlap, nonspecific binding, available conjugation chemistries, and cellular toxicity. A complementary or alternative, label-free approach to molecular information is through multiplex coherent anti-Stokes Raman scattering (MCARS), which is a coherent, nonlinear optical method that provides a wealth of molecular information by probing the Raman energies within a molecule. In this work, we demonstrate the unique capability of our MCARS flow cytometer to distinguish flowing particles and discuss system performance capabilities and possibilities.

© 2009 Optical Society of America

OCIS Codes
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:
Nonlinear Optics

Original Manuscript: October 19, 2009
Revised Manuscript: November 13, 2009
Manuscript Accepted: November 20, 2009
Published: November 30, 2009

Virtual Issues
Vol. 5, Iss. 1 Virtual Journal for Biomedical Optics

Charles H. Camp, Siva Yegnanarayanan, Ali A. Eftekhar, Hamsa Sridhar, and Ali Adibi, "Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry," Opt. Express 17, 22879-22889 (2009)

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