OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 5 — Mar. 2, 2009
  • pp: 4095–4111

Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources

Barry Cense, Erik Koperda, Jeffrey M. Brown, Omer P. Kocaoglu, Weihua Gao, Ravi S. Jonnal, and Donald T. Miller  »View Author Affiliations


Optics Express, Vol. 17, Issue 5, pp. 4095-4111 (2009)
http://dx.doi.org/10.1364/OE.17.004095


View Full Text Article

Acrobat PDF (987 KB) ISP Components
Browse Datasets: MIDAS Click for help

Full-Text PDF contains links to datasets. See ISP homepage for software requirements and other information.





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Ultrabroadband sources, such as multiplexed superluminescent diodes (SLDs) and femtosecond lasers, have been successfully employed in adaptive optics optical coherence tomography (AO-OCT) systems for ultrahigh resolution retinal imaging. The large cost differential of these sources, however, motivates the need for a performance comparison. Here, we compare the performance of a Femtolasers Integral Ti:Sapphire laser and a Superlum BroadLighter T840, using the same AO-OCT system and the same subject. In addition, we investigate the capability of our instrument equipped with the Integral to capture volume images of the fovea and adjacent regions on a second subject using the AO to control focus in the retina and custom and freeware image registration software to reduce eye motion artifacts. Monochromatic ocular aberrations were corrected with a woofer-tweeter AO system. Coherence lengths of the Integral and BroadLighter were measured in vivo at 3.2 µm and 3.3 µm, respectively. The difference in dynamic range was 5 dB, close to the expected variability of the experiment. Individual cone photoreceptors, retinal capillaries and nerve fiber bundles were distinguished in all three dimensions with both sources. The acquired retinal volumes are provided for viewing in OSA ISP, allowing the reader to data mine at the microscope level.

© 2009 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(170.4470) Medical optics and biotechnology : Ophthalmology
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(330.4300) Vision, color, and visual optics : Vision system - noninvasive assessment

ToC Category:
OCT Technology

History
Original Manuscript: October 8, 2008
Revised Manuscript: January 7, 2009
Manuscript Accepted: January 19, 2009
Published: March 2, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics
Interactive Science Publishing Focus Issue: Optical Coherence Tomography (OCT) (2009) Optics Express

Citation
Barry Cense, Erik Koperda, Jeffrey M. Brown, Omer P. Kocaoglu, Weihua Gao, Ravi S. Jonnal, and Donald T. Miller, "Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources," Opt. Express 17, 4095-4111 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-5-4095


Sort:  Author  |  Year  |  Journal  |  Reset

References

  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254,1178-1181 (1991). [CrossRef]
  2. W. Drexler, U. Morgner, R. K. Ghanta, F. X. Kartner, J. S. Schuman, and J. G. Fujimoto, "Ultrahigh-resolution ophthalmic optical coherence tomography," Nat. Med. 7,502-507 (2001). [CrossRef]
  3. M. Wojtkowski, R. Leitgeb, A. Kowalczyk, T. Bajraszewski, and A. F. Fercher, "In vivo human retinal imaging by Fourier domain optical coherence tomography," J. Biomed. Opt. 7,457-463 (2002). [CrossRef]
  4. T. Mitsui, "Dynamic range of optical reflectometry with spectral interferometry," Jpn. J. Appl. Phys. Part 1, 38,6133-6137 (1999). [CrossRef]
  5. R. Leitgeb, C. K. Hitzenberger, and A. F. Fercher, "Performance of fourier domain vs. time domain optical coherence tomography," Opt. Express 11,889-894 (2003).
  6. J. F. de Boer, B. Cense, B. H. Park, M. C. Pierce, G. J. Tearney, and B. E. Bouma, "Improved signal-to-noise ratio in spectral-domain compared with time-domain optical coherence tomography," Opt. Lett. 28,2067-2069 (2003). [CrossRef]
  7. B. Cense, N. A. Nassif, T. C. Chen, M. C. Pierce, S. H. Yun, B. H. Park, B. E. Bouma, G. J. Tearney, and J. F. de Boer, "Ultrahigh-resolution high-speed retinal imaging using spectral-domain optical coherence tomography," Opt. Express 12,2435-2447 (2004). [CrossRef]
  8. M. Wojtkowski, V. J. Srinivasan, T. H. Ko, J. G. Fujimoto, A. Kowalczyk, and J. S. Duker, "Ultrahigh-resolution, high-speed, Fourier domain optical coherence tomography and methods for dispersion compensation," Opt. Express 12,2404-2422 (2004). [CrossRef]
  9. J. Porter, H. Queener, J. Lin, K. E. Thorn, and A. Awwal, Adaptive Optics for Vision Science: Principles, Practices, Design and Applications (Wiley, 2006).
  10. Y. Zhang, J. T. Rha, R. S. Jonnal, and D. T. Miller, "Adaptive optics parallel spectral domain optical coherence tomography for imaging the living retina," Opt. Express 13,4792-4811 (2005). [CrossRef]
  11. R. J. Zawadzki, S. M. Jones, S. S. Olivier, M. T. Zhao, B. A. Bower, J. A. Izatt, S. Choi, S. Laut, and J. S. Werner, "Adaptive-optics optical coherence tomography for high-resolution and high-speed 3D retinal in vivo imaging," Opt. Express 13,8532-8546 (2005). [CrossRef]
  12. E. J. Fernandez, B. Povazay, B. Hermann, A. Unterhuber, H. Sattmann, P. M. Prieto, R. Leitgeb, P. Ahnelt, P. Artal, and W. Drexler, "Three-dimensional adaptive optics ultrahigh-resolution optical coherence tomography using a liquid crystal spatial light modulator," Vision Res. 45,3432-3444 (2005). [CrossRef]
  13. Y. Zhang, B. Cense, J. Rha, R. S. Jonnal, W. Gao, R. J. Zawadzki, J. S. Werner, S. Jones, S. Olivier, and D. T. Miller, "High-speed volumetric imaging of cone photoreceptors with adaptive optics spectral-domain optical coherence tomography," Opt. Express 14,4380-4394 (2006). [CrossRef]
  14. C. E. Bigelow, N. V. Iftimia, R. D. Ferguson, T. E. Ustun, B. Bloom, and D. X. Hammer, "Compact multimodal adaptive-optics spectral-domain optical coherence tomography instrument for retinal imaging," J. Opt. Soc. Am. A 24,1327-1336 (2007). [CrossRef]
  15. R. J. Zawadzki, S. S. Choi, S. M. Jones, S. S. Oliver, and J. S. Werner, "Adaptive optics-optical coherence tomography: optimizing visualization of microscopic retinal structures in three dimensions," J. Opt. Soc. Am. A 24,1373-1383 (2007). [CrossRef]
  16. R. J. Zawadzki, B. Cense, Y. Zhang, S. S. Choi, D. T. Miller, and J. S. Werner, "Ultrahigh-resolution optical coherence tomography with monochromatic and chromatic aberration correction," Opt. Express 16,8126-8143 (2008). [CrossRef]
  17. E. J. Fernandez, B. Hermann, B. Povazay, A. Unterhuber, H. Sattmann, B. Hofer, P. K. Ahnelt, and W. Drexler, "Ultrahigh resolution optical coherence tomography and pancorrection for cellular imaging of the living human retina," Opt. Express 16,11083-11094 (2008). [CrossRef]
  18. D. C. Chen, S. M. Jones, D. A. Silva, and S. S. Olivier, "High-resolution adaptive optics scanning laser ophthalmoscope with dual deformable mirrors," J. Opt. Soc. Am. A 24,1305-1312 (2007). [CrossRef]
  19. E. J. Fernandez, L. Vabre, B. Hermann, A. Unterhuber, B. Povazay, and W. Drexler, "Adaptive optics with a magnetic deformable mirror: applications in the human eye," Opt. Express 14,8900-8917 (2006). [CrossRef]
  20. N. A. Nassif, B. Cense, B. H. Park, M. C. Pierce, S. H. Yun, B. E. Bouma, G. J. Tearney, T. C. Chen, and J. F. de Boer, "In vivo high-resolution video-rate spectral-domain optical coherence tomography of the human retina and optic nerve," Opt. Express 12,367-376 (2004). [CrossRef]
  21. M. Mujat, B. H. Park, B. Cense, T. C. Chen, and J. F. de Boer, "Autocalibration of spectral-domain optical coherence tomography spectrometers for in vivo quantitative retinal nerve fiber layer birefringence determination," J. Biomed. Opt. 12,041205 (2007). [CrossRef]
  22. F. C. Delori, R. H. Webb, and D. H. Sliney, "Maximum permissible exposures for ocular safety (ANSI 2000), with emphasis on ophthalmic devices," J. Opt. Soc. Am. A 24,1250-1265 (2007). [CrossRef]
  23. A.N.S.I., "Z136.1 Safe use of lasers" (Laser Institute of America, 2007).
  24. M. D. Abramoff, P. J. Magalhaes, and S. J. Ram, "Image processing with ImageJ," Biophotonics Int. 11,36-43 (2004).
  25. P. Thevenaz, U. E. Ruttimann, and M. Unser, "A pyramid approach to subpixel registration based on intensity," IEEE Trans. Image Process. 7,27-41 (1998). [CrossRef]
  26. P. Bedggood, M. Daaboul, R. Ashman, G. Smith, and A. Metha, "Characteristics of the human isoplanatic patch and implications for adaptive optics retinal imaging," J. Biomed. Opt. 13,024008 (2008). [CrossRef]
  27. C. A. Curcio, K. R. Sloan, R. E. Kalina, and A. E. Hendrickson, "Human photoreceptor topography," J. Comp. Neurol. 292,497-523 (1990). [CrossRef]
  28. W. Bloom, and D. W. Fawcett, A Textbook of Histology (W. B. Saunders Company, 1975).
  29. M. J. Hogan, J. A. Alvarado, and J. Esperson Weddell, Histology of the Human Eye (W. B. Saunders Company, 1971).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Multimedia

Multimedia FilesRecommended Software
» Media 1: AVI (80350 KB)      QuickTime

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited