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Optics Express

Optics Express

  • Vol. 17, Iss. 7 — Mar. 30, 2009
  • pp: 5075–5082

Evanescent-wave fluorescence microscopy using symmetric planar waveguides

Björn Agnarsson, Saevar Ingthorsson, Thorarinn Gudjonsson, and Kristjan Leosson  »View Author Affiliations

Optics Express, Vol. 17, Issue 7, pp. 5075-5082 (2009)

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We describe a new evanescent-wave fluorescence excitation method, ideally suited for imaging of biological samples. The excitation light propagates in a planar optical waveguide, consisting of a thin waveguide core sandwiched between a sample in an aqueous solution and a polymer with a matching refractive index, forming a symmetric cladding environment. This configuration offers clear advantages over other waveguide-excitation methods, such as superior image quality, wide tunability of the evanescent field penetration depth and compatibility with optical fibers. The method is well suited for cell membrane imaging on cells in culture, including cell-cell and cell-matrix interaction, monitoring of surface binding events and similar applications involving aqueous solutions.

© 2009 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(230.7390) Optical devices : Waveguides, planar
(130.5460) Integrated optics : Polymer waveguides

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 27, 2009
Revised Manuscript: March 10, 2009
Manuscript Accepted: March 11, 2009
Published: March 16, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics

Bjorn Agnarsson, Saevar Ingthorsson, Thorarinn Gudjonsson, and Kristjan Leosson, "Evanescent-wave fluorescence microscopy using symmetric planar waveguides," Opt. Express 17, 5075-5082 (2009)

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