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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 8 — Apr. 13, 2009
  • pp: 5987–5997

Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

Ruei-Yu He, Yuan-Deng Su, Keng-Chi Cho, Chun-Yun Lin, Nan-Shan Chang, Chih-Han Chang, and Shean-Jen Chen  »View Author Affiliations

Optics Express, Vol. 17, Issue 8, pp. 5987-5997 (2009)

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A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide fluorescent images of living cell membranes. The proposed microscope with the help of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having a shorter fluorophore lifetime. In comparison with a one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. By combining the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated it’s capability for brighter and more contrasted fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

© 2009 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(240.6680) Optics at surfaces : Surface plasmons
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 6, 2009
Revised Manuscript: March 22, 2009
Manuscript Accepted: March 22, 2009
Published: March 30, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Ruei-Yu He, Yuan-Deng Su, Keng-Chi Cho, Chun-Yun Lin, Nan-Shan Chang, Chih-Han Chang, and Shean-Jen Chen, "Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging," Opt. Express 17, 5987-5997 (2009)

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