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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 8 — Apr. 13, 2009
  • pp: 6867–6880

Annular pupil filter under shot-noise condition for linear and non linear microscopy

Emiliano Ronzitti, Giuseppe Vicidomini, Valentina Caorsi, and Alberto Diaspro  »View Author Affiliations

Optics Express, Vol. 17, Issue 8, pp. 6867-6880 (2009)

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The imaging performances of multiphoton excitation and confocal laser scanning microscopy are herby considered: in typical experimental imaging conditions, a small finite amount of photon reaches the detector giving shot-noise fluctuations which affects the signal acquired. A significant detriment in the high frequencies transmission capability is obtained. In order to partially recover the high frequencies information lost, the insertion of a pupil plane filter in the microscope illumination light pathway on the objective lens is proposed. We demonstrate high-frequency and resolution enhancement in the case of linear and non linear fluorescence microscope approach under shot-noise condition.

© 2009 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: January 21, 2009
Revised Manuscript: March 12, 2009
Manuscript Accepted: March 15, 2009
Published: April 10, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Emiliano Ronzitti, Giuseppe Vicidomini, Valentina Caorsi, and Alberto Diaspro, "Annular pupil filter under shot-noise condition for linear and non linear microscopy," Opt. Express 17, 6867-6880 (2009)

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