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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 13 — Jun. 21, 2010
  • pp: 13708–13719

Stimulated Raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses

Yasuyuki Ozeki, Yuma Kitagawa, Kazuhiko Sumimura, Norihiko Nishizawa, Wataru Umemura, Shin’ichiro Kajiyama, Kiichi Fukui, and Kazuyoshi Itoh  »View Author Affiliations


Optics Express, Vol. 18, Issue 13, pp. 13708-13719 (2010)
http://dx.doi.org/10.1364/OE.18.013708


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Abstract

We propose and demonstrate the use of subharmonically synchronized laser pulses for low-noise lock-in detection in stimulated Raman scattering (SRS) microscopy. In the experiment, Yb-fiber laser pulses at a repetition rate of 38 MHz are successfully synchronized to Ti:sapphire laser pulses at a repetition rate of 76 MHz with a jitter of <8 fs by a two-photon detector and an intra-cavity electro-optic modulator. By using these pulses, high-frequency lock-in detection of SRS signal is accomplished without high-speed optical modulation. The noise level of the lock-in signal is found to be higher than the shot noise limit only by 1.6 dB. We also demonstrate high-contrast, 3D imaging of unlabeled living cells.

© 2010 OSA

OCIS Codes
(320.7090) Ultrafast optics : Ultrafast lasers
(180.5655) Microscopy : Raman microscopy

ToC Category:
Microscopy

History
Original Manuscript: May 10, 2010
Revised Manuscript: June 3, 2010
Manuscript Accepted: June 6, 2010
Published: June 10, 2010

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Yasuyuki Ozeki, Yuma Kitagawa, Kazuhiko Sumimura, Norihiko Nishizawa, Wataru Umemura, Shin’ichiro Kajiyama, Kiichi Fukui, and Kazuyoshi Itoh, "Stimulated Raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses," Opt. Express 18, 13708-13719 (2010)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-18-13-13708


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