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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 18 — Aug. 30, 2010
  • pp: 19339–19352

Polarization-resolved Second Harmonic microscopy in anisotropic thick tissues

Ivan Gusachenko, Gaël Latour, and Marie-Claire Schanne-Klein  »View Author Affiliations

Optics Express, Vol. 18, Issue 18, pp. 19339-19352 (2010)

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We thoroughly analyze the linear propagation effects that affect polarization-resolved Second Harmonic Generation imaging of thick anisotropic tissues such as collagenous tissues. We develop a theoretical model that fully accounts for birefringence and diattenuation along the excitation propagation, and polarization scrambling upon scattering of the harmonic signal. We obtain an excellent agreement with polarization-resolved SHG images at increasing depth within a rat-tail tendon for both polarizations of the forward SHG signal. Most notably, we observe interference fringes due to birefringence in the SHG depth profile when excited at π/4 angle from the tendon axis. We also measure artifactual decrease of ρ = χxxx /χxyy with depth due to diattenuation of the excitation. We therefore derive a method that proves reliable to determine both ρ and the tendon birefringence and diattenuation.

© 2010 Optical Society of America

OCIS Codes
(120.5410) Instrumentation, measurement, and metrology : Polarimetry
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(190.2620) Nonlinear optics : Harmonic generation and mixing
(180.4315) Microscopy : Nonlinear microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:

Original Manuscript: July 13, 2010
Revised Manuscript: August 20, 2010
Manuscript Accepted: August 22, 2010
Published: August 26, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Ivan Gusachenko, Gaël Latour, and Marie-Claire Schanne-Klein, "Polarization-resolved Second Harmonic microscopy in anisotropic thick tissues," Opt. Express 18, 19339-19352 (2010)

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