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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 6 — Mar. 15, 2010
  • pp: 5390–5398

A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection

Jesse D. McMullen and Warren R. Zipfel  »View Author Affiliations

Optics Express, Vol. 18, Issue 6, pp. 5390-5398 (2010)

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We present a de novo design of an objective for use in multi-photon (MPM) and second harmonic generation (SHG) microscopy. This objective was designed to have a large field of view (FOV), while maintaining a moderate numerical aperture (NA) and relative straight forward construction. A dichroic beam splitter was incorporated within the objective itself allowing for an increase in the front aperture of the objective and corresponding enhancement of the solid angle of collected emission by an order of magnitude over existing designs.

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OCIS Codes
(110.2970) Imaging systems : Image detection systems
(170.0110) Medical optics and biotechnology : Imaging systems
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(290.1350) Scattering : Backscattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: December 18, 2009
Revised Manuscript: January 22, 2010
Manuscript Accepted: February 1, 2010
Published: March 1, 2010

Virtual Issues
Vol. 5, Iss. 7 Virtual Journal for Biomedical Optics

Jesse D. McMullen and Warren R. Zipfel, "A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection," Opt. Express 18, 5390-5398 (2010)

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