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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 18, Iss. 7 — Mar. 29, 2010
  • pp: 7210–7218

High spatiotemporal resolution imaging of fast intrinsic optical signals activated by retinal flicker stimulation

Yang-Guo Li, Qiu-Xiang Zhang, Lei Liu, Franklin R. Amthor, and Xin-Cheng Yao  »View Author Affiliations

Optics Express, Vol. 18, Issue 7, pp. 7210-7218 (2010)

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High resolution monitoring of stimulus-evoked retinal neural activities is important for understanding retinal neural mechanisms, and can be a powerful tool for retinal disease diagnosis and treatment outcome evaluation. Fast intrinsic optical signals (IOSs), which have the time courses comparable to that of electrophysiological activities in the retina, hold the promise for high resolution imaging of retinal neural activities. However, application of fast IOS imaging has been hindered by the contamination of slow, high magnitude optical responses associated with transient hemodynamic and metabolic changes. In this paper we demonstrate the feasibility of separating fast retinal IOSs from slow optical responses by combining flicker stimulation and dynamic (temporal) differential image processing. A near infrared flood-illumination microscope equipped with a high-speed (1000 Hz) digital camera was used to conduct concurrent optical imaging and ERG measurement of isolated frog retinas. High spatiotemporal resolution imaging revealed that fast IOSs could follow flicker frequency up to at least 6 Hz. Comparable time courses of fast IOSs and ERG kinetics provide evidence that fast IOSs are originated from stimulus activated retinal neurons.

© 2010 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(330.4270) Vision, color, and visual optics : Vision system neurophysiology
(330.5310) Vision, color, and visual optics : Vision - photoreceptors
(330.5380) Vision, color, and visual optics : Physiology

ToC Category:
Vision, Color, and Visual Optics

Original Manuscript: December 16, 2009
Revised Manuscript: February 11, 2010
Manuscript Accepted: February 23, 2010
Published: March 24, 2010

Virtual Issues
Vol. 5, Iss. 7 Virtual Journal for Biomedical Optics

Yang-Guo Li, Qiu-Xiang Zhang, Lei Liu, Franklin R. Amthor, and Xin-Cheng Yao, "High spatiotemporal resolution imaging of fast intrinsic optical signals activated by retinal flicker stimulation," Opt. Express 18, 7210-7218 (2010)

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