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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 1 — Jan. 3, 2011
  • pp: 387–398

Microscope-based label-free microfluidic cytometry

Xuantao Su, Sean E. Kirkwood, Manisha Gupta, Leah Marquez-Curtis, Yuanyuan Qiu, Anna Janowska-Wieczorek, Wojciech Rozmus, and Ying Y. Tsui  »View Author Affiliations


Optics Express, Vol. 19, Issue 1, pp. 387-398 (2011)
http://dx.doi.org/10.1364/OE.19.000387


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Abstract

A microscope-based label-free microfluidic cytometer capable of acquiring two dimensional light scatter patterns from single cells, pattern analysis of which determines cellular information such as cell size, orientation and inner nanostructure, was developed. Finite-difference time-domain numerical simulations compared favorably with experimental scatter patterns from micrometer-sized beads and cells. The device was capable of obtaining light scattering patterns from the smallest mature blood cells (platelets) and cord blood hematopoietic stem/progenitor cells (CD34 + cells) and myeloid precursor cells. The potential for evaluation of cells using this label-free microfluidic cytometric technique was discussed.

© 2010 OSA

OCIS Codes
(000.4430) General : Numerical approximation and analysis
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(290.0290) Scattering : Scattering

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: September 27, 2010
Revised Manuscript: December 8, 2010
Manuscript Accepted: December 12, 2010
Published: December 24, 2010

Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Xuantao Su, Sean E. Kirkwood, Manisha Gupta, Leah Marquez-Curtis, Yuanyuan Qiu, Anna Janowska-Wieczorek, Wojciech Rozmus, and Ying Y. Tsui, "Microscope-based label-free microfluidic cytometry," Opt. Express 19, 387-398 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-1-387


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References

  1. B. K. Patterson, M. Till, P. Otto, C. Goolsby, M. R. Furtado, L. J. McBride, and S. M. Wolinsky, “Detection of HIV-1 DNA and messenger RNA in individual cells by PCR-driven in situ hybridization and flow cytometry,” Science 260(5110), 976–979 (1993). [CrossRef] [PubMed]
  2. H. M. Shapiro, Practical Flow Cytometry, (John Wiley & Sons, Inc., Hoboken, NJ, 2003).
  3. P. O. Krutzik and G. P. Nolan, “Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling,” Nat. Methods 3(5), 361–368 (2006). [CrossRef] [PubMed]
  4. D. Psaltis, S. R. Quake, and C. H. Yang, “Developing optofluidic technology through the fusion of microfluidics and optics,” Nature 442(7101), 381–386 (2006). [CrossRef] [PubMed]
  5. C. Monat, P. Domachuk, and B. J. Eggleton, “Integrated optofluidics: A new river of light,” Nat. Photonics 1(2), 106–114 (2007). [CrossRef]
  6. M. M. Wang, E. Tu, D. E. Raymond, J. M. Yang, H. C. Zhang, N. Hagen, B. Dees, E. M. Mercer, A. H. Forster, I. Kariv, P. J. Marchand, and W. F. Butler, “Microfluidic sorting of mammalian cells by optical force switching,” Nat. Biotechnol. 23(1), 83–87 (2005). [CrossRef]
  7. X. T. Su, C. Capjack, W. Rozmus, and C. Backhouse, “2D light scattering patterns of mitochondria in single cells,” Opt. Express 15(17), 10562–10575 (2007). [CrossRef] [PubMed]
  8. X. T. Su, K. Singh, C. Capjack, J. Petrácek, C. Backhouse, and W. Rozmus, “Measurements of light scattering in an integrated microfluidic waveguide cytometer,” J. Biomed. Opt. 13(2), 024024 (2008). [CrossRef] [PubMed]
  9. H. Craighead, “Future lab-on-a-chip technologies for interrogating individual molecules,” Nature 442(7101), 387–393 (2006). [CrossRef] [PubMed]
  10. R. Drezek, A. Dunn, and R. Richards-Kortum, “A pulsed finite-difference time-domain (FDTD) method for calculating light scattering from biological cells over broad wavelength ranges,” Opt. Express 6(7), 147–157 (2000). [CrossRef] [PubMed]
  11. H. Subramanian, P. Pradhan, Y. Liu, I. R. Capoglu, X. Li, J. D. Rogers, A. Heifetz, D. Kunte, H. K. Roy, A. Taflove, and V. Backman, “Optical methodology for detecting histologically unapparent nanoscale consequences of genetic alterations in biological cells,” Proc. Natl. Acad. Sci. U.S.A. 105(51), 20118–20123 (2008). [CrossRef] [PubMed]
  12. G. C. Salzman, M. E. Wilder, and J. H. Jett, “Light scattering with stream-in-air flow systems,” J. Histochem. Cytochem. 27(1), 264–267 (1979). [CrossRef] [PubMed]
  13. M. Bartholdi, G. C. Salzman, R. D. Hiebert, and M. Kerker, “Differential light scattering photometer for rapid analysis of single particles in flow,” Appl. Opt. 19(10), 1573–1581 (1980). [CrossRef] [PubMed]
  14. J. D. Wilson, C. E. Bigelow, D. J. Calkins, and T. H. Foster, “Light scattering from intact cells reports oxidative-stress-induced mitochondrial swelling,” Biophys. J. 88(4), 2929–2938 (2005). [CrossRef] [PubMed]
  15. E. Maurer-Spurej, K. Brown, A. Labrie, A. Marziali, and O. Glatter, “Portable dynamic light scattering instrument and method for the measurement of blood platelet suspensions,” Phys. Med. Biol. 51(15), 3747–3758 (2006). [CrossRef] [PubMed]
  16. Z. Wang, J. El-Ali, M. Engelund, T. Gotsaed, I. R. Perch-Nielsen, K. B. Mogensen, D. Snakenborg, J. P. Kutter, and A. Wolff, “Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements,” Lab Chip 4(4), 372–377 (2004). [CrossRef] [PubMed]
  17. C. McGuckin, M. Jurga, H. Ali, M. Strbad, and N. Forraz, “Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro,” Nat. Protoc. 3(6), 1046–1055 (2008). [CrossRef] [PubMed]
  18. X. T. Su, S. E. Kirkwood, H. Gul, K. Singh, M. Z. Islam, A. Janowska-Wieczorek, W. Rozmus, and Y. Y. Tsui, “Light scattering characterization of single biological cells in a microfluidic cytometer,” Proc. SPIE 7386, 738602, 738602-8 (2009). [CrossRef]
  19. K. M. Jacobs, J. Q. Lu, and X. H. Hu, “Development of a diffraction imaging flow cytometer,” Opt. Lett. 34(19), 2985–2987 (2009). [CrossRef] [PubMed]
  20. A. Taflove, Computational Electrodynamics: The Finite-Difference Time-Domain Method, (Artech House, Inc., Norwood, Massachusetts, 1995).
  21. A. Dunn and R. Richards-Kortum, “Three-dimensional computation of light scattering from cells,” IEEE J. Sel. Top. Quantum Electron. 2(4), 898–905 (1996). [CrossRef]
  22. X. Li, A. Taflove, and V. Backman, “Recent progress in exact and reduced-order modeling of light-scattering properties of complex structures,” IEEE J. Sel. Top. Quantum Electron. 11(4), 759–765 (2005). [CrossRef]
  23. C. G. Liu, C. Capjack, and W. Rozmus, “3-D simulation of light scattering from biological cells and cell differentiation,” J. Biomed. Opt. 10(1), 014007 (2005). [CrossRef]
  24. J. Q. Lu, P. Yang, and X. H. Hu, “Simulations of light scattering from a biconcave red blood cell using the finite-difference time-domain method,” J. Biomed. Opt. 10(2), 024022 (2005). [CrossRef] [PubMed]
  25. M. Majka, A. Janowska-Wieczorek, J. Ratajczak, K. Ehrenman, Z. Pietrzkowski, M. A. Kowalska, A. M. Gewirtz, S. G. Emerson, and M. Z. Ratajczak, “Numerous growth factors, cytokines, and chemokines are secreted by human CD34(+) cells, myeloblasts, erythroblasts, and megakaryoblasts and regulate normal hematopoiesis in an autocrine/paracrine manner,” Blood 97(10), 3075–3085 (2001). [CrossRef] [PubMed]
  26. L. Marquez-Curtis, A. Jalili, K. Deiteren, N. Shirvaikar, A. M. Lambeir, and A. Janowska-Wieczorek, “Carboxypeptidase M expressed by human bone marrow cells cleaves the C-terminal lysine of stromal cell-derived factor-1alpha: another player in hematopoietic stem/progenitor cell mobilization?” Stem Cells 26(5), 1211–1220 (2008). [CrossRef] [PubMed]
  27. D. Watson, N. Hagen, J. Diver, P. Marchand, and M. Chachisvilis, “Elastic light scattering from single cells: orientational dynamics in optical trap,” Biophys. J. 87(2), 1298–1306 (2004). [CrossRef] [PubMed]
  28. R. De, A. Zemel, and S. A. Safran, “Dynamics of cell orientation,” Nat. Phys. 3(9), 655–659 (2007). [CrossRef]
  29. M. D. Abramoff, P. J. Magelhaes, and S. J. Ram, “Image Processing with ImageJ,” Biophoton. Int. 11, 36–42 (2004).

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