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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 1 — Jan. 3, 2011
  • pp: 99–106

Intrinsic optical signal imaging of glucose-stimulated insulin secreting β-cells

Yi-Chao Li, Wan-Xing Cui, Xu-Jing Wang, Franklin Amthor, Rong-Wen Lu, Anthony Thompson, and Xin-Cheng Yao  »View Author Affiliations

Optics Express, Vol. 19, Issue 1, pp. 99-106 (2011)

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Simultaneous monitoring of many functioning β-cells is essential for understanding β-cell dysfunction as an early event in the progression to diabetes. Intrinsic optical signal (IOS) imaging has been shown to allow high resolution detection of stimulus-evoked physiological responses in the retina and other neural tissues. In this paper, we demonstrate the feasibility of using IOS imaging for functional examination of insulin secreting INS-1 cells, a popular model for investigating diabetes associated β-cell dysfunction. Our experiments indicate that IOS imaging permits simultaneous monitoring of glucose-stimulated physiological responses in multiple cells with high spatial (sub-cellular) and temporal (sub-second) resolution. Rapid IOS image sequences revealed transient optical responses that had time courses tightly correlated with the glucose stimulation.

© 2011 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(330.5380) Vision, color, and visual optics : Physiology
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 4, 2010
Revised Manuscript: November 12, 2010
Manuscript Accepted: November 17, 2010
Published: December 21, 2010

Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics

Yi-Chao Li, Wan-Xing Cui, Xu-Jing Wang, Franklin Amthor, Rong-Wen Lu, Anthony Thompson, and Xin-Cheng Yao, "Intrinsic optical signal imaging of glucose-stimulated insulin secreting β-cells," Opt. Express 19, 99-106 (2011)

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