OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 21 — Oct. 10, 2011
  • pp: 19822–19835

Three dimension optical imaging in a high throughput layered expression system

Reut Cohen, Gallya Gannot, Moshe Ben David, Nir Sochen, and Israel Gannot  »View Author Affiliations

Optics Express, Vol. 19, Issue 21, pp. 19822-19835 (2011)

View Full Text Article

Enhanced HTML    Acrobat PDF (1316 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Layered peptide array (LPA) system enables multiplex screening of biomarkers [1-3]. One of the main problems of the LPA system is the screening of the layered-membranes stack. Nowadays, each membrane is imaged separately using conventional fluorescent imaging. This process is time consuming and requires extensive manual interaction. This paper describes a general solution for optical imaging of a layered grid medium using photogrammetric methods. The suggested method enables visualization of the LPA membranes stack by using only two images of the stack. This study is a proof of concept of the suggested solution using MATLAB simulation and phantom experiments.

© 2011 OSA

OCIS Codes
(150.6910) Machine vision : Three-dimensional sensing
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 21, 2011
Revised Manuscript: August 16, 2011
Manuscript Accepted: August 17, 2011
Published: September 26, 2011

Virtual Issues
Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics

Reut Cohen, Gallya Gannot, Moshe Ben David, Nir Sochen, and Israel Gannot, "Three dimension optical imaging in a high throughput layered expression system," Opt. Express 19, 19822-19835 (2011)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007). [CrossRef] [PubMed]
  2. G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005). [CrossRef] [PubMed]
  3. G. Gannot, M. A. Tangrea, A. M. Richardson, M. J. Flaig, S. M. Hewitt, E. M. Marcus, M. R. Emmert-Buck, and R. F. Chuaqui, “Layered expression scanning: multiplex molecular analysis of diverse life science platforms,” Clin. Chim. Acta 376(1-2), 9–16 (2007). [CrossRef] [PubMed]
  4. H. Mitchell and I. Newton, “Medical photogrammetric measurement: overview and prospects,” ISPRS J. Photogramm. Remote Sens. 56(5-6), 286–294 (2002). [CrossRef]
  5. J. Bouguet, “Camera calibration toolbox for matlab,” (2004).
  6. J. Heikkila and O. Silven, “A four-step camera calibration procedure with implicit image correction,” in (Citeseer, 1997), 1106–1112.
  7. R. Cohen, Device and Method for Imaging of Layered Medium for Multiplex Screening of Mmarkers in Cancer Tssue Biopsies (Tel Aviv University, 2010).
  8. P. Rosin, “Unimodal thresholding,” Pattern Recognit. 34(11), 2083–2096 (2001). [CrossRef]
  9. G. W. Zack, W. E. Rogers, and S. A. Latt, “Automatic measurement of sister chromatid exchange frequency,” J. Histochem. Cytochem. 25(7), 741–753 (1977). [CrossRef] [PubMed]
  10. L. Vincent, “Morphological grayscale reconstruction in image analysis: applications and efficient algorithms,” IEEE Trans. Image Process. 2(2), 176–201 (1993). [CrossRef] [PubMed]
  11. F. Meyer and S. Beucher, “Morphological segmentation,” J. Vis. Commun. Image Represent. 1(1), 21–46 (1990). [CrossRef]
  12. Y. Ma, S. Soatto, and J. Koeck An invitation to 3-D vision: From images to geometric models (Springer Verlag, 2004).
  13. A. Fusiello, E. Trucco, and A. Verri, “A compact algorithm for rectification of stereo pairs,” Mach. Vis. Appl. 12(1), 16–22 (2000). [CrossRef]
  14. R. Westaway, S. Lane, and D. Hicks, “Remote sensing of clear-water, shallow, gravel-bed rivers using digital photogrammetry,” Photogramm. Eng. Remote Sensing 67, 1271–1282 (2001).
  15. J. MacQueen, “Some methods for classification and analysis of multivariate observations,” in (California, USA, 1967), 14.
  16. H. Maas, A. Gruen, and D. Papantoniou, “Particle tracking velocimetry in three-dimensional flows,” Exp. Fluids 15(2), 133–146 (1993). [CrossRef]
  17. A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited