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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 22 — Oct. 24, 2011
  • pp: 21627–21642

Fast parallel interferometric 3D tracking of numerous optically trapped particles and their hydrodynamic interaction

Dominic Ruh, Benjamin Tränkle, and Alexander Rohrbach  »View Author Affiliations

Optics Express, Vol. 19, Issue 22, pp. 21627-21642 (2011)

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Multi-dimensional, correlated particle tracking is a key technology to reveal dynamic processes in living and synthetic soft matter systems. In this paper we present a new method for tracking micron-sized beads in parallel and in all three dimensions – faster and more precise than existing techniques. Using an acousto-optic deflector and two quadrant-photo-diodes, we can track numerous optically trapped beads at up to tens of kHz with a precision of a few nanometers by back-focal plane interferometry. By time-multiplexing the laser focus, we can calibrate individually all traps and all tracking signals in a few seconds and in 3D. We show 3D histograms and calibration constants for nine beads in a quadratic arrangement, although trapping and tracking is easily possible for more beads also in arbitrary 2D arrangements. As an application, we investigate the hydrodynamic coupling and diffusion anomalies of spheres trapped in a 3 × 3 arrangement.

© 2011 OSA

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(140.7010) Lasers and laser optics : Laser trapping
(260.3160) Physical optics : Interference
(290.0290) Scattering : Scattering

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: July 14, 2011
Revised Manuscript: August 16, 2011
Manuscript Accepted: August 22, 2011
Published: October 19, 2011

Virtual Issues
Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics

Dominic Ruh, Benjamin Tränkle, and Alexander Rohrbach, "Fast parallel interferometric 3D tracking of numerous optically trapped particles and their hydrodynamic interaction," Opt. Express 19, 21627-21642 (2011)

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