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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 23 — Nov. 7, 2011
  • pp: 22364–22376

Long-range and long-term interferometric tracking by static and dynamic force-clamp optical tweezers.

A. Guiggiani, B. Torre, A. Contestabile, F. Benfenati, M. Basso, M. Vassalli, and F. Difato  »View Author Affiliations

Optics Express, Vol. 19, Issue 23, pp. 22364-22376 (2011)

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Optical tweezers are recognized single-molecule technique to resolve forces and motion on the molecular scale. Complex biological phenomena, such as cell differentiation and locomotion, require long range tracking capabilities with nanometer resolution over an extended period, to resolve molecular processes on the cellular scale. Here we introduce a real-time control of the microscope stage position to perform long-term tracking, with sub-millisecond resolution, of a bead attached to a neuron, preserving sub-nanometer sensitivity on a spatial range of centimeters, seven orders of magnitude larger. Moreover, the suitability of the system is tested by time- modulating the force-clamp condition to study the role of statically and dynamically applied forces in neuronal differentiation.

© 2011 OSA

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis
(350.4855) Other areas of optics : Optical tweezers or optical manipulation
(120.4880) Instrumentation, measurement, and metrology : Optomechanics

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: August 8, 2011
Revised Manuscript: September 10, 2011
Manuscript Accepted: September 10, 2011
Published: October 24, 2011

Virtual Issues
Vol. 7, Iss. 1 Virtual Journal for Biomedical Optics

A. Guiggiani, B. Torre, A. Contestabile, F. Benfenati, M. Basso, M. Vassalli, and F. Difato, "Long-range and long-term interferometric tracking by static and dynamic force-clamp optical tweezers.," Opt. Express 19, 22364-22376 (2011)

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