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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 23 — Nov. 7, 2011
  • pp: 22643–22658

Photo-physiological variability in phytoplankton chlorophyll fluorescence and assessment of chlorophyll concentration

Alexander Chekalyuk and Mark Hafez  »View Author Affiliations

Optics Express, Vol. 19, Issue 23, pp. 22643-22658 (2011)

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Photo-physiological variability of in vivo chlorophyll fluorescence (CF) per unit of chlorophyll concentration (CC) is analyzed using a biophysical model to improve the accuracy of CC assessments. Field measurements of CF and photosystem II (PSII) photochemical yield (PY) with the Advanced Laser Fluorometer (ALF) in the Delaware and Chesapeake Bays are analyzed vs. high-performance liquid chromatography (HPLC) CC retrievals. It is shown that isolation from ambient light, PSII saturating excitation, optimized phytoplankton exposure to excitation, and phytoplankton dark adaptation may provide accurate in vivo CC fluorescence measurements (R2 = 0.90–0.95 vs. HPLC retrievals). For in situ or flow-through measurements that do not allow for dark adaptation, concurrent PY measurements can be used to adjust for CF non-photochemical quenching (NPQ) and improve the accuracy of CC fluorescence assessments. Field evaluation has shown the NPQ-invariance of CF/PY and CF(PY−1-1) parameters and their high correlation with HPLC CC retrievals (R2 = 0.74–0.96), while the NPQ-affected CF measurements correlated poorly with CC (R2 = −0.22).

© 2011 OSA

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(140.0140) Lasers and laser optics : Lasers and laser optics
(300.0300) Spectroscopy : Spectroscopy
(280.4788) Remote sensing and sensors : Optical sensing and sensors

ToC Category:
Atmospheric and Oceanic Optics

Original Manuscript: September 1, 2011
Revised Manuscript: October 7, 2011
Manuscript Accepted: October 8, 2011
Published: October 25, 2011

Virtual Issues
Vol. 7, Iss. 1 Virtual Journal for Biomedical Optics

Alexander Chekalyuk and Mark Hafez, "Photo-physiological variability in phytoplankton chlorophyll fluorescence and assessment of chlorophyll concentration," Opt. Express 19, 22643-22658 (2011)

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  50. A. M. Chekalyuk, Lamont Doherty Earth Observatory of Columbia University, 61 Route 9W, Palisades, NY 10964, M. Landry, R. Goericke, A. G. Taylor, and M. Hafez are preparing a manuscript to be called “Laser fluorescence phytoplankton analysis across a frontal zone in the California Current Ecosystem.”

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