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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 3 — Jan. 31, 2011
  • pp: 2546–2555

Design and application of a confocal microscope for spectrally resolved anisotropy imaging

Alessandro Esposito, Arjen N. Bader, Simon C. Schlachter, Dave J. van den Heuvel, Gabriele S. Kaminski Schierle, Ashok R. Venkitaraman, Clemens F. Kaminski, and Hans C. Gerritsen  »View Author Affiliations

Optics Express, Vol. 19, Issue 3, pp. 2546-2555 (2011)

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Biophysical imaging tools exploit several properties of fluorescence to map cellular biochemistry. However, the engineering of a cost-effective and user-friendly detection system for sensing the diverse properties of fluorescence is a difficult challenge. Here, we present a novel architecture for a spectrograph that permits integrated characterization of excitation, emission and fluorescence anisotropy spectra in a quantitative and efficient manner. This sensing platform achieves excellent versatility of use at comparatively low costs. We demonstrate the novel optical design with example images of plant cells and of mammalian cells expressing fluorescent proteins undergoing energy transfer.

© 2011 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.1790) Microscopy : Confocal microscopy
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:

Original Manuscript: November 16, 2010
Revised Manuscript: January 17, 2011
Manuscript Accepted: January 23, 2011
Published: January 26, 2011

Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics

Alessandro Esposito, Arjen N. Bader, Simon C. Schlachter, Dave J. van den Heuvel, Gabriele S. Kaminski Schierle, Ashok R. Venkitaraman, Clemens F. Kaminski, and Hans C. Gerritsen, "Design and application of a confocal microscope for spectrally resolved anisotropy imaging," Opt. Express 19, 2546-2555 (2011)

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