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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 5 — Feb. 28, 2011
  • pp: 3890–3901

Dynamic tissue analysis using time- and wavelength-resolved fluorescence spectroscopy for atherosclerosis diagnosis

Yinghua Sun, Yang Sun, Douglas Stephens, Hongtao Xie, Jennifer Phipps, Ramez Saroufeem, Jeffrey Southard, Daniel S. Elson, and Laura Marcu  »View Author Affiliations


Optics Express, Vol. 19, Issue 5, pp. 3890-3901 (2011)
http://dx.doi.org/10.1364/OE.19.003890


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Abstract

Simultaneous time- and wavelength-resolved fluorescence spectroscopy (STWRFS) was developed and tested for the dynamic characterization of atherosclerotic tissue ex vivo and arterial vessels in vivo. Autofluorescence, induced by a 337 nm, 700 ps pulsed laser, was split to three wavelength sub-bands using dichroic filters, with each sub-band coupled into a different length of optical fiber for temporal separation. STWRFS allows for fast recording/analysis (few microseconds) of time-resolved fluorescence emission in these sub-bands and rapid scanning. Distinct compositions of excised human atherosclerotic aorta were clearly discriminated over scanning lengths of several centimeters based on fluorescence lifetime and the intensity ratio between 390 and 452 nm. Operation of STWRFS blood flow was further validated in pig femoral arteries in vivo using a single-fiber probe integrated with an ultrasound imaging catheter. Current results demonstrate the potential of STWRFS as a tool for real-time optical characterization of arterial tissue composition and for atherosclerosis research and diagnosis.

© 2011 OSA

OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.6500) Spectroscopy : Spectroscopy, time-resolved

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 30, 2010
Revised Manuscript: January 19, 2011
Manuscript Accepted: February 8, 2011
Published: February 14, 2011

Virtual Issues
Vol. 6, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Yinghua Sun, Yang Sun, Douglas Stephens, Hongtao Xie, Jennifer Phipps, Ramez Saroufeem, Jeffrey Southard, Daniel S. Elson, and Laura Marcu, "Dynamic tissue analysis using time- and wavelength-resolved fluorescence spectroscopy for atherosclerosis diagnosis," Opt. Express 19, 3890-3901 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-5-3890


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