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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 6 — Mar. 14, 2011
  • pp: 5047–5062

Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy

Gary Brooker, Nisan Siegel, Victor Wang, and Joseph Rosen  »View Author Affiliations


Optics Express, Vol. 19, Issue 6, pp. 5047-5062 (2011)
http://dx.doi.org/10.1364/OE.19.005047


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Abstract

Fresnel Incoherent Correlation Holography (FINCH) enables holograms and 3D images to be created from incoherent light with just a camera and spatial light modulator (SLM). We previously described its application to microscopic incoherent fluorescence wherein one complex hologram contains all the 3D information in the microscope field, obviating the need for scanning or serial sectioning. We now report experiments which have led to the optimal optical, electro-optic, and computational conditions necessary to produce holograms which yield high quality 3D images from fluorescent microscopic specimens. An important improvement from our previous FINCH configurations capitalizes on the polarization sensitivity of the SLM so that the same SLM pixels which create the spherical wave simulating the microscope tube lens, also pass the plane waves from the infinity corrected microscope objective, so that interference between the two wave types at the camera creates a hologram. This advance dramatically improves the resolution of the FINCH system. Results from imaging a fluorescent USAF pattern and a pollen grain slide reveal resolution which approaches the Rayleigh limit by this simple method for 3D fluorescent microscopic imaging.

© 2011 OSA

OCIS Codes
(050.1950) Diffraction and gratings : Diffraction gratings
(090.1760) Holography : Computer holography
(090.1970) Holography : Diffractive optics
(090.2880) Holography : Holographic interferometry
(100.6890) Image processing : Three-dimensional image processing
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(120.5060) Instrumentation, measurement, and metrology : Phase modulation
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(260.2510) Physical optics : Fluorescence
(330.1720) Vision, color, and visual optics : Color vision
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: December 13, 2010
Revised Manuscript: February 22, 2011
Manuscript Accepted: February 27, 2011
Published: March 2, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Gary Brooker, Nisan Siegel, Victor Wang, and Joseph Rosen, "Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy," Opt. Express 19, 5047-5062 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-6-5047


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References

  1. B. W. Schilling, T.-C. Poon, G. Indebetouw, B. Storrie, K. Shinoda, Y. Suzuki, and M. H. Wu, “Three-dimensional holographic fluorescence microscopy,” Opt. Lett. 22(19), 1506–1508 (1997). [CrossRef]
  2. J. Rosen and G. Brooker, “Non-Scanning Motionless Fluorescence Three-Dimensional Holographic Microscopy,” Nat. Photonics 2(3), 190–195 (2008). [CrossRef]
  3. O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010). [CrossRef] [PubMed]
  4. E. Y. Lam, X. Zhang, H. Vo, T.-C. Poon, and G. Indebetouw, “Three-dimensional microscopy and sectional image reconstruction using optical scanning holography,” Appl. Opt. 48(34), H113–H119 (2009). [CrossRef] [PubMed]
  5. G. Indebetouw, Y. Tada, J. Rosen, and G. Brooker, “Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms,” Appl. Opt. 46(6), 993–1000 (2007). [CrossRef] [PubMed]
  6. J. Rosen and G. Brooker, “Digital spatially incoherent Fresnel holography,” Opt. Lett. 32(8), 912–914 (2007). [CrossRef] [PubMed]
  7. J. Rosen and G. Brooker, “Fluorescence incoherent color holography,” Opt. Express 15(5), 2244–2250 (2007). [CrossRef] [PubMed]
  8. J. W. Goodman, Introduction to Fourier Optics, 2nd ed. (McGraw-Hill, 1996).
  9. C. Maurer, A. Jesacher, S. Bernet, and M. Ritsch-Marte, “What spatial light modulators can do for optical microscopy,” Laser Photonics Rev. 5(1), 81–101 (2011). [CrossRef]
  10. I. Yamaguchi and T. Zhang, “Phase-shifting digital holography,” Opt. Lett. 22(16), 1268–1270 (1997). [CrossRef] [PubMed]
  11. B. Katz, D. Wulich, and J. Rosen, “Optimal noise suppression in Fresnel incoherent correlation holography (FINCH) configured for maximum imaging resolution,” Appl. Opt. 49(30), 5757–5763 (2010). [CrossRef] [PubMed]
  12. G. D. Love, “Wave-front correction and production of Zernike modes with a liquid-crystal spatial light modulator,” Appl. Opt. 36(7), 1517–1520 (1997). [CrossRef] [PubMed]

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