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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 19, Iss. 9 — Apr. 25, 2011
  • pp: 8066–8072

STED nanoscopy with mass-produced laser diodes

Susanne Schrof, Thorsten Staudt, Eva Rittweger, Nina Wittenmayer, Thomas Dresbach, Johann Engelhardt, and Stefan W. Hell  »View Author Affiliations


Optics Express, Vol. 19, Issue 9, pp. 8066-8072 (2011)
http://dx.doi.org/10.1364/OE.19.008066


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Abstract

We show that far-field fluorescence nanoscopy by stimulated emission depletion (STED) can be realized with compact off-the-shelf laser diodes, such as those used in laser pointers and DVDs. A spatial resolution of 40-50 nm is attained by pulsing a 660 nm DVD-diode. The efficacy of these low-cost STED microscopes in biological imaging is demonstrated by differentiating between clusters of the synaptic protein bassoon and transport vesicles in hippocampal neurons, based on the feature diameter. Our results facilitate the implementation of this all-molecular-transition based superresolution method in many applications ranging from nanoscale fluorescence imaging to nanoscale fluorescence sensing.

© 2011 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(140.2020) Lasers and laser optics : Diode lasers
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: February 7, 2011
Revised Manuscript: March 29, 2011
Manuscript Accepted: April 6, 2011
Published: April 12, 2011

Virtual Issues
Vol. 6, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Susanne Schrof, Thorsten Staudt, Eva Rittweger, Nina Wittenmayer, Thomas Dresbach, Johann Engelhardt, and Stefan W. Hell, "STED nanoscopy with mass-produced laser diodes," Opt. Express 19, 8066-8072 (2011)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-9-8066


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References

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