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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 11 — May. 21, 2012
  • pp: 12166–12170

Multi-contrast focal modulation microscopy for in vivo imaging of thick biological tissues

Nanguang Chen and Guangjun Gao  »View Author Affiliations


Optics Express, Vol. 20, Issue 11, pp. 12166-12170 (2012)
http://dx.doi.org/10.1364/OE.20.012166


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Abstract

In vivo high resolution imaging of biological tissues is desirable for a wide range of biomedical applications. Recently focal modulation microscopy (FMM) has been developed and an imaging depth comparable to multi-photon microscopy (MPM) and optical coherence microscopy (OCM) has been achieved. Here we report the first focal modulation microscope that is capable of performing real-time fluorescence and scattering imaging simultaneously on thick biological tissues. A novel spatiotemporal phase modulator (STPM) has been designed and integrated into such a microscope to achieve high performances in terms of imaging speed, contrast, effective spatial resolution, signal to noise ratio, and compatibility with multiple excitation wavelengths.

© 2012 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0110) Medical optics and biotechnology : Imaging systems
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 26, 2012
Revised Manuscript: May 9, 2012
Manuscript Accepted: May 10, 2012
Published: May 14, 2012

Virtual Issues
Vol. 7, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Nanguang Chen and Guangjun Gao, "Multi-contrast focal modulation microscopy for in vivo imaging of thick biological tissues," Opt. Express 20, 12166-12170 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-11-12166


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