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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 13 — Jun. 18, 2012
  • pp: 14451–14459

Photonic crystal slabs for surface contrast enhancement in microscopy of transparent objects

Yousef Nazirizadeh, Tim Becker, Julia Reverey, Christine Selhuber-Unkel, Daniel H. Rapoport, Uli Lemmer, and Martina Gerken  »View Author Affiliations


Optics Express, Vol. 20, Issue 13, pp. 14451-14459 (2012)
http://dx.doi.org/10.1364/OE.20.014451


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Abstract

In optical microscopy the contrast of transparent objects achieved with conventional methods is often not satisfactory, for example for the automated recognition of cells. In this paper we present a nano-optical label-free approach for contrast enhancement based on photonic crystal slabs (PCS) as the specimen holder. Quasi-guided modes inside these structures cause an intrinsic color of the PCS, which strongly depends on the wavelength and the quality factor of the optical mode. Objects on the surface of the PCS experience a significant color and intensity contrast enhancement, as they change properties of the optical modes.

© 2012 OSA

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(160.5298) Materials : Photonic crystals

ToC Category:
Microscopy

History
Original Manuscript: February 21, 2012
Revised Manuscript: May 4, 2012
Manuscript Accepted: May 5, 2012
Published: June 13, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Yousef Nazirizadeh, Tim Becker, Julia Reverey, Christine Selhuber-Unkel, Daniel H. Rapoport, Uli Lemmer, and Martina Gerken, "Photonic crystal slabs for surface contrast enhancement in microscopy of transparent objects," Opt. Express 20, 14451-14459 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-13-14451


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