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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 14 — Jul. 2, 2012
  • pp: 16039–16049

PALMER: a method capable of parallel localization of multiple emitters for high-density localization microscopy

Yina Wang, Tingwei Quan, Shaoqun Zeng, and Zhen-Li Huang  »View Author Affiliations


Optics Express, Vol. 20, Issue 14, pp. 16039-16049 (2012)
http://dx.doi.org/10.1364/OE.20.016039


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Abstract

Developing methods for high-density localization of multiple emitters is a promising approach for enhancing the temporal resolution of localization microscopy while maintaining a desired spatial resolution, but the widespread use of this approach is thus far mainly obstructed by the slow image analysis speed. Here we present a high-density localization method based on the combination of Graphics Processing Unit (GPU) parallel computation, multiple-emitter fitting, and model recommendation via Bayesian Information Criterion (BIC). This method, called PALMER, exhibits satisfactory localization accuracy comparable with the previous reported SSM_BIC method, while executes more than two orders of magnitudes faster. Meanwhile, compared to the conventional localization microscopy which is based on sparse emitter localization, high-density localization microscopy based the PALMER method allows a speed gain of up to ~14-fold in obtaining a super-resolution image with the same Nyquist resolution.

© 2012 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(110.2960) Imaging systems : Image analysis
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Image Processing

History
Original Manuscript: April 26, 2012
Revised Manuscript: June 13, 2012
Manuscript Accepted: June 21, 2012
Published: June 29, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Yina Wang, Tingwei Quan, Shaoqun Zeng, and Zhen-Li Huang, "PALMER: a method capable of parallel localization of multiple emitters for high-density localization microscopy," Opt. Express 20, 16039-16049 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-14-16039


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