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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 14 — Jul. 2, 2012
  • pp: 16077–16082

Phase contrast microscopy with fringe contrast adjustable by using grating-based phase-shifter

Juanjuan Zheng, Baoli Yao, Peng Gao, and Tong Ye  »View Author Affiliations


Optics Express, Vol. 20, Issue 14, pp. 16077-16082 (2012)
http://dx.doi.org/10.1364/OE.20.016077


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Abstract

In this paper a new phase contrast method with fringe contrast adjustable is proposed. In the Fourier plane of the object wave, two Ronchi gratings i.e., a central grating and a surrounding grating, are used to modulate the phases of the undiffracted and diffracted components, respectively. By loading the two gratings separately on spatial light modulator, the undiffracted and diffracted components can be measured independently, which simplify greatly the reconstruction process. Besides, the fringe contrast of the phase contrast interferogram can be adjusted by changing the modulation depth of the two gratings. The feasibility of the proposed method is verified by theoretical analysis and experiment.

© 2012 OSA

OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(100.5090) Image processing : Phase-only filters
(170.0180) Medical optics and biotechnology : Microscopy

ToC Category:
Microscopy

History
Original Manuscript: May 10, 2012
Revised Manuscript: June 22, 2012
Manuscript Accepted: June 23, 2012
Published: June 29, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Juanjuan Zheng, Baoli Yao, Peng Gao, and Tong Ye, "Phase contrast microscopy with fringe contrast adjustable by using grating-based phase-shifter," Opt. Express 20, 16077-16082 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-14-16077


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