Sharper fluorescent super-resolution spot generated by azimuthally polarized beam in STED microscopy

doi:10.1364/OE.20.017653

Sharper fluorescent super-resolution spot generated by azimuthally polarized beam in STED microscopy

Yi Xue, Cuifang Kuang, Shuai Li, Zhaotai Gu, and Xu Liu »View Author Affiliations

Optics Express, Vol. 20, Issue 16, pp. 17653-17666 (2012)
http://dx.doi.org/10.1364/OE.20.017653

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Abstract

A novel method is proposed for generating sharper fluorescent super-resolution spot by azimuthally polarized beam in stimulated emission depletion (STED) microscopy. The incoherent superposition of azimuthally polarized beam with five-zone binary phase plate and the same beam with quadrant $0 / π$ phase plate can yield a tightly focused doughnut spot surrounded completely and uniformly. And azimuthally polarized beam modulated by a vortex 0—2π phase plate works as pump beam. Compared with known effective excitation spot yielded by circular polarized STED beam, the azimuthally polarized beam result is shaper, as well as energy-saving, costing only ~50% of the energy cost by circular polarized beam. A STED beam of less intensity has the potential to reduce fluorescence photobleaching and photodamage in living cell imaging. In addition, the influence of E_z absence as well as FWHM of pump beam in the focal field is discussed.

OCIS Codes
(050.1380) Diffraction and gratings : Binary optics
(100.6640) Image processing : Superresolution
(180.2520) Microscopy : Fluorescence microscopy
(260.5430) Physical optics : Polarization

ToC Category:
Microscopy

History
Original Manuscript: April 30, 2012
Revised Manuscript: July 7, 2012
Manuscript Accepted: July 13, 2012
Published: July 19, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Yi Xue, Cuifang Kuang, Shuai Li, Zhaotai Gu, and Xu Liu, "Sharper fluorescent super-resolution spot generated by azimuthally polarized beam in STED microscopy," Opt. Express 20, 17653-17666 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-16-17653

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References

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