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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 20 — Sep. 24, 2012
  • pp: 22585–22601

Optimal tracking of a Brownian particle

Alexander P. Fields and Adam E. Cohen  »View Author Affiliations


Optics Express, Vol. 20, Issue 20, pp. 22585-22601 (2012)
http://dx.doi.org/10.1364/OE.20.022585


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Abstract

Optical tracking of a fluorescent particle in solution faces fundamental constraints due to Brownian motion, diffraction, and photon shot noise. Background photons and imperfect tracking apparatus further degrade tracking precision. Here we use a model of particle motion to combine information from multiple time-points to improve the localization precision. We derive successive approximations that enable real-time particle tracking with well controlled tradeoffs between precision and computational cost. We present the theory in the context of feedback electrokinetic trapping, though the results apply to optical tracking of any particle subject to diffusion and drift. We use numerical simulations and experimental data to validate the algorithms’ performance.

© 2012 OSA

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(110.3055) Imaging systems : Information theoretical analysis
(110.4155) Imaging systems : Multiframe image processing

ToC Category:
Microscopy

History
Original Manuscript: August 2, 2012
Revised Manuscript: September 11, 2012
Manuscript Accepted: September 13, 2012
Published: September 18, 2012

Virtual Issues
Vol. 7, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Alexander P. Fields and Adam E. Cohen, "Optimal tracking of a Brownian particle," Opt. Express 20, 22585-22601 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-20-22585


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