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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 23 — Nov. 5, 2012
  • pp: 25378–25390

Polarization-based non-staining cell detection

M. Zhang, K. Ihida-Stansbury, J. Van der Spiegel, and N. Engheta  »View Author Affiliations


Optics Express, Vol. 20, Issue 23, pp. 25378-25390 (2012)
http://dx.doi.org/10.1364/OE.20.025378


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Abstract

Polarization is an important characteristic of electromagnetic waves, which can not be detected by either the human visual system or traditional image sensors. Motivated by various animal species with polarization vision as well as by the prospect of improving the image quality of the imaging systems, we are exploring the potential of polarization for microscope imaging. The most powerful techniques for molecule monitoring requires complex preprocessing for labeling the sample with different dyes. In this paper, we propose a cell detection method using polarization imaging without any need for staining target cell samples with any chemical dye. The motivation for this work is to develop an optical imaging technique that is simple and that can be used on live cells. The polarization sensitivity of cell samples is studied in this paper. A definition for the quantity called “polarization deviation” is proposed in order to identify clearer the difference between target cells and the background. Based on the polarization deviation detection method, a three-parameter polarization imaging method is employed to further simplify the image capture procedure for the proposed label-free cell detection. A color imaging methodology based on the well-known color space is utilized in order to represent the captured polarization information using computer graphics.

© 2012 OSA

OCIS Codes
(100.0100) Image processing : Image processing
(230.5440) Optical devices : Polarization-selective devices
(260.5430) Physical optics : Polarization

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 6, 2012
Revised Manuscript: October 18, 2012
Manuscript Accepted: October 18, 2012
Published: October 24, 2012

Virtual Issues
Vol. 7, Iss. 12 Virtual Journal for Biomedical Optics

Citation
M. Zhang, K. Ihida-Stansbury, J. Van der Spiegel, and N. Engheta, "Polarization-based non-staining cell detection," Opt. Express 20, 25378-25390 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-23-25378


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References

  1. D. Stephens, Cell Imaging (Scion, 2006).
  2. H. J. Conn, Biological Stains: A Handbook on the Nature and Uses of the Dyes Employed in the Biological Laboratory (Biotech Publications, 1953).
  3. G. Clark, Staining Procedures (Williams and Wilkins, 1981).
  4. G. Clark, F. H. Kasten, and H. J. Conn, History of Staining (Williams and Wilkins, 1981).
  5. F. Zernike, “Phase contrast, a new method for the microscopic observation of transparent objects,” Physica9, 686–698 (1942). [CrossRef]
  6. F. Zernike, “Phasecontrast, a new method for the microscopicobservation of transparentobjects part II,” Physica9, 974–980 (1942). [CrossRef]
  7. D. Goldstein, “Polarized Light” (Marcel Dekker: New York, 2003). [CrossRef]
  8. T. H. Waterman, “Polarization sensitivity” in The Handbook of Sensory Physiology, vol. VII/6B Vision in Invertebrates, edited by H. Autrum, (Springer-Verlag, New York, 1981). [CrossRef]
  9. R. Wehner, “Polarized-light navigation by insects,” SCI AM235, 106–114, (1976). [CrossRef] [PubMed]
  10. T. Labhart, “Polarization opponent interneurons in the insect visual system,” Nature331, 435–437 (1988). [CrossRef]
  11. R. Wehner, “Neurobiology of polarization vision,” TRENDS NEUROSCI12, 353–359, (1989). [CrossRef] [PubMed]
  12. T. W. Cronin and J. Marshall, “Parallel processing and image analysis in the eyes of mantis shrimps,” Biol. Bull.200, 177–183 (2001). [CrossRef] [PubMed]
  13. S. G. Demos, W. B. Wang, and R. R. Alfano, “Imaging objects hidden in scattering media with fluorescence polarization preservation of contrast agents,” Appl. Opt.37, 792–797 (1998). [CrossRef]
  14. J. G. Walker, P. C. Chang, and K. I. Hopcraft, “Visibility depth improvement in active polarization imaging in scattering media,” Appl. Opt.39, 4933–4941 (2000). [CrossRef]
  15. H. H. Wang, C. W. Sun, Y. M. Wang, Y. W. Kiang, and C. C. Yang, “Determination of the depth of a scattering target in a turbid medium with polarization discrimination of transmitted signals,” Opt. Lett.28, 25–27 (2003). [CrossRef] [PubMed]
  16. P. C. Y. Chang, J. C. Flitton, K. I. Hopcraft, E. Jakeman, D. L. Jordan, and J. G. Walker, “Improving visibility depth in passive underwater imaging by use of polarization,” Appl. Opt.42, 2794–803 (2003). [CrossRef] [PubMed]
  17. Y. Y. Schechner, N. Karpel, T. Israel, and I. Technology, “Clear Underwater Vision,” Pattern Recogn1, 536–543 (2004).
  18. Y. Y. Schechner and N. Karpel, “Recovery of Underwater Visibility and Structure by Polarization Analysis,” IEEE J Oceanic Eng.30, 570–587 (2005). [CrossRef]
  19. T. Treibitz and Y. Y. Schechner, “Active polarization descattering,” IEEE Trans. Pattern Anal. Mach. Intell.31, 385–399 (2009). [CrossRef] [PubMed]
  20. S. Shwartz, E. Namer, and Y. Schechner, “Blind Haze Seperation,” in Proceedings of IEEE Computer Society Conference on Computer Vision and Pattern Recognition (IEEE2006), pp. 1984–1991.
  21. J. S. Tyo, M. P. Rowe, E. N. Pugh, and N. Engheta, “Target detection in optically scatter media by polarization-difference imaging”, Appl. Opt.35, 1855–1870 (1996). [CrossRef] [PubMed]
  22. B. Lyot, Research on the Polarization of Light from Planets and Some Substances (Ann. Observatoire de Paris, Section de Meudon1929).
  23. W. G. Egan, W. R. Johnson, and V. S. Whitehead, “Terrestrial polarization imagery obtained from the Space Shuttle: characterization and interpretation,” Appl. Opt.30, 435–42 (1991). [CrossRef] [PubMed]
  24. J. L. Deuz, F. M. Bron, C. Devaux, P. Goloub, M. Herman, B. Lafrance, F. Maignan, A. Marchand, F. Nadal, G. Perry, and D. Tanr, “Remote sensing of aerosols over land surfaces from POLDER-ADEOS-1 polarized measurements”, J. Geophys. Res.106, 4913–4926 (2001). [CrossRef]
  25. J. S. Tyo, D. L. Goldstein, D. B. Chenault, and J. A. Shaw, “Review of passive imaging polarimetry for remote sensing applications,” Appl. Opt.45, 5453–5469 (2006). [CrossRef] [PubMed]
  26. S. S. Lin, K. M. Yemelyanov, E. N. Pugh, and N. Engheta, “Polarization-based and specular-reflection-based noncontact latent fingerprint imaging and lifting,” J. Opt. Soc. Am. A23, 2137–2153 (2006). [CrossRef]
  27. S. S. Lin, K. M. Yemelyanov, E. N. Pugh, and N. Engheta, “Separation and contrast enhancement of overlapping cast shadow components using polarization,” Opt. Express14, 7099–7108 (2006). [CrossRef] [PubMed]
  28. Y. Y. Schechner, J. Shamir, and N. Kiryati, “Vision through semireflecting media: polarization analysis,” Opt. Lett.24, 1088–1090 (1999). [CrossRef]
  29. T. Germer and M. Fasolka, “Characterizing surface roughness of thin films by polarized light,” Proc. SPIE518, 264–275 (2003). [CrossRef]
  30. D. Miyazaki, R. Tan, K. Hara, and K. Ikeuchi, “Polarization-based Inverse Rendering from a Single View,” in Proceedings of Ninth IEEE International Conference on Computer Vision (IEEE2003), pp. 982–987. [CrossRef]
  31. F. Goudail and P. Rfrgier, “Statistical algorithms for target detection in coherent active polarimetric images,” J. Opt. Soc. Am. A18, 3049–3060 (2001). [CrossRef]
  32. F. Goudail and P. Rfrgier, “Statistical techniques for target detection in polarization diversity images,” Opt. Lett.26, 644–646 (2001). [CrossRef]
  33. F. Goudail, P. Terrier, Y. Takakura, L. Bigu, F. Galland, and V. DeVlaminck, “Target detection with a liquid-crystal-based passive Stokes polarimeter.,” Appl. Opt.43, 274–82 (2004). [CrossRef] [PubMed]
  34. R. Nothdurft and G. Yao, “Expression of target optical properties in subsurface polarization-gated imaging,” Opt. Express13, 4185–4195 (2005). [CrossRef] [PubMed]
  35. R. C. Gonzalez and P. Wintz, “Image Enhancement,” in Digital Image Processing (Addison Weslwy, 1987), pp. 146–152.
  36. G. D. Bernard and R. Wehner, “Functional similarities between polarization vision and color vision,” Vision Res.17, 1019–1028 (1977). [CrossRef] [PubMed]
  37. M. P. Rowe, E. N. Pugh, and N. Engheta, “Polarizationdifference imaging: a biologically inspired technique for observation through scattering media,” Opt. Lett.20, 608–610 (1995). [CrossRef] [PubMed]
  38. J. S. Tyo, M. P. Rowe, E. N. Pugh, and N. Engheta, “Target detection in optically scattering media by polarization-difference imaging,” Appl. Opt.35, 1855–1870 (1996). [CrossRef] [PubMed]
  39. J. S. Tyo, E. N. Pugh, and N. Engheta, “Colorimetric representation for use with polarization-difference imaging of objects in scattering media,” J. Opt. Soc. Am. A15, 367–374 (1998). [CrossRef]
  40. S.-S. Lin, K. M. Yemelyanov, E. N. Pugh, and N. Engheta, “Separation and contrast enhancement of overlapping cast shadow components using polarization,” Opt. Express14, 7099–7108 (2006). [CrossRef] [PubMed]
  41. S.-S. Lin, K. M. Yemelyanov, E. N. Pugh, and N. Engheta, “Polarization-based and specular-reflection-based noncontact latent fingerprint imaging and lifting,” J. Opt. Soc. Am. A23, 2137–2153 (2006). [CrossRef]
  42. K. M. Yemelyanov, S. S. Lin, W. Q. Luis, E. N. Pugh, and N. Engheta, “Bio-inspired display of polarization information using selected visual cues,” Proc. of SPIE5158, 71–84 (2003). [CrossRef]

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