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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 23 — Nov. 5, 2012
  • pp: 25700–25710

Structured illumination microscopy for in-vivo human retinal imaging: a theoretical assessment

Sabah Chetty and Steve Gruppetta  »View Author Affiliations

Optics Express, Vol. 20, Issue 23, pp. 25700-25710 (2012)

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Structured illumination microscopy applied to in-vivo retinal imaging has the potential to provide a low-cost and powerful diagnostic tool for retinal disease. In this paper the key parameters that affect performance in structured illumination ophthalmoscopy are studied theoretically. These include the number of images that need to be acquired in order to generate a sectioned image, which is affected by the non-stationary nature of the retina during acquisition, the choice of spatial frequency of the illuminating sinusoid, the effect of typical ocular aberrations on axial resolution and the nature of the sinusoidal pattern produced by the illumination system. The results indicate that structured illumination ophthalmoscopy can be a robust technique for achieving axial sectioning in retinal imaging without the need for complex optical systems.

© 2012 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.4460) Medical optics and biotechnology : Ophthalmic optics and devices
(170.4470) Medical optics and biotechnology : Ophthalmology
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 5, 2012
Revised Manuscript: October 15, 2012
Manuscript Accepted: October 17, 2012
Published: October 29, 2012

Virtual Issues
Vol. 7, Iss. 12 Virtual Journal for Biomedical Optics

Sabah Chetty and Steve Gruppetta, "Structured illumination microscopy for in-vivo human retinal imaging: a theoretical assessment," Opt. Express 20, 25700-25710 (2012)

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