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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 28 — Dec. 31, 2012
  • pp: 29369–29377

Rotating-polarization CARS microscopy: combining chemical and molecular orientation sensitivity

Giuseppe de Vito, Angelo Bifone, and Vincenzo Piazza  »View Author Affiliations

Optics Express, Vol. 20, Issue 28, pp. 29369-29377 (2012)

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Coherent Anti-Stokes Raman Spectroscopy (CARS) is a non-linear process in which the energy difference of a pair of incoming photons matches the energy of the vibrational mode of a molecular bond of interest. This phonon population is coherently probed by a third photon and anti-Stokes radiation is emitted. Here a novel approach to CARS microscopy is presented yielding the intensity of the anti-Stokes emission, the directionality the molecular bonds of interest, and their average orientation. Myelinated axons in fixed mouse-brain slices have been imaged by RP-CARS. We were able to detect the local average direction of the acylic chains of membrane phospholipids and their spatial anisotropy. This novel method may impact the study of healthy brain circuitry as well as demyelinating diseases or other pathological states associated with altered neural connectivity.

© 2012 OSA

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy
(180.5655) Microscopy : Raman microscopy

ToC Category:

Original Manuscript: October 26, 2012
Revised Manuscript: November 30, 2012
Manuscript Accepted: November 30, 2012
Published: December 18, 2012

Virtual Issues
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics

Giuseppe de Vito, Angelo Bifone, and Vincenzo Piazza, "Rotating-polarization CARS microscopy: combining chemical and molecular orientation sensitivity," Opt. Express 20, 29369-29377 (2012)

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