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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 20, Iss. 28 — Dec. 31, 2012
  • pp: 29378–29385

Optics InfoBase > Optics Express > Volume 20 > Issue 28 > Page 29378

Molecular beacons immobilized within suspended core optical fiber for specific DNA detection

Linh Viet Nguyen, Stephen C. Warren-Smith, Alan Cooper, and Tanya M. Monro  »View Author Affiliations


Optics Express, Vol. 20, Issue 28, pp. 29378-29385 (2012)
http://dx.doi.org/10.1364/OE.20.029378


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Abstract

We propose and experimentally demonstrate a new class of sensor for specific DNA sequences based on molecular beacons (MB) immobilized on the internal surfaces of suspended core optical fibers (SCF). MBs, a type of hairpin structured DNA probe, are attached on the surface of the SCF core using a fuzzy nanoassembly process used in conjunction with a biotin-streptavidin-biotin surface attachment strategy. The proposed DNA sensor detects complementary DNA sequences (cDNA) while discriminating sequences differing from the target by just one base. This enables the detection of DNA in unprecedentedly small sample volumes (nL scale) and is, to the best of our knowledge, the first specific DNA detection using a DNA probe immobilized within a microstructured optical fiber.

© 2012 OSA

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(060.4005) Fiber optics and optical communications : Microstructured fibers

ToC Category:
Fiber Optics and Optical Communications

History
Original Manuscript: November 1, 2012
Revised Manuscript: December 3, 2012
Manuscript Accepted: December 3, 2012
Published: December 18, 2012

Virtual Issues
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Linh Viet Nguyen, Stephen C. Warren-Smith, Alan Cooper, and Tanya M. Monro, "Molecular beacons immobilized within suspended core optical fiber for specific DNA detection," Opt. Express 20, 29378-29385 (2012)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-20-28-29378


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References

  1. O. S. Wolfbeis, “Fiber-Optic Chemical Sensors and Biosensors,” Anal. Chem.78(12), 3859–3874 (2006). [CrossRef] [PubMed]
  2. L. Rindorf, J. B. Jensen, M. Dufva, L. H. Pedersen, P. E. Høiby, and O. Bang, “Photonic crystal fiber long-period gratings for biochemical sensing,” Opt. Express14(18), 8224–8231 (2006). [CrossRef] [PubMed]
  3. H. S. Jang, K. N. Park, J. P. Kim, S. J. Sim, O. J. Kwon, Y.-G. Han, and K. S. Lee, “Sensitive DNA biosensor based on a long-period grating formed on the side-polished fiber surface,” Opt. Express17(5), 3855–3860 (2009). [CrossRef] [PubMed]
  4. X. Chen, L. Zhang, K. Zhou, E. Davies, K. Sugden, I. Bennion, M. Hughes, and A. Hine, “Real-time detection of DNA interactions with long-period fiber-grating-based biosensor,” Opt. Lett.32(17), 2541–2543 (2007). [CrossRef] [PubMed]
  5. Y. Wang, K. L. Cooper, and A. Wang, “Microgap structure optical sensor for fast label-free DNA detection,” J. Lightwave Technol.26(17), 3181–3185 (2008). [CrossRef]
  6. Y. Yanina, Shevchenko, David A. Blair, Maria C. Derosa, and Jacques Albert, “DNA Target Detection Using Gold-Coated Tilted Fiber Bragg Gratings in Aqueous Media,” in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, Technical Digest (CD) (Optical Society of America, 2008), paper CMJ4.
  7. D. R. Walt, “Fibre Optic Microarrays,” Chem. Soc. Rev.39(1), 38–50 (2009). [CrossRef] [PubMed]
  8. S. Sforza, R. Corradini, T. Tedeschi, and R. Marchelli, “Food analysis and food authentication by peptide nucleic acid (PNA)-based technologies,” Chem. Soc. Rev.40(1), 221–232 (2010). [CrossRef] [PubMed]
  9. J. C. Knight, J. Broeng, T. A. Birks, and P. S. J. Russell, “Photonic band gap guidance in optical fibers,” Science282(5393), 1476–1478 (1998). [CrossRef] [PubMed]
  10. T. M. Monro, S. C. Warren-Smith, E. P. Schartner, A. François, S. Heng, H. Ebendorff-Heidepriem, and S. Afshar, “Sensing with suspended-core optical fibers,” Opt. Fiber Technol.16(6), 343–356 (2010). [CrossRef]
  11. Y. Ruan, T. C. Foo, S. C. Warren-Smith, P. Hoffmann, R. C. Moore, H. Ebendorff-Heidepriem, and T. M. Monro, “Antibody immobilization within glass microstructured fibers: a route to sensitive and selective biosensors,” Opt. Express16(22), 18514–18523 (2008). [CrossRef] [PubMed]
  12. S. C. Warren-Smith, S. Heng, H. Ebendorff-Heidepriem, A. D. Abell, and T. M. Monro, “Fluorescence-based aluminum ion sensing using a surface-functionalized microstructured optical fiber,” Langmuir27(9), 5680–5685 (2011). [CrossRef] [PubMed]
  13. S. C. Warren-Smith, E. Sinchenko, P. R. Stoddart, and T. M. Monro, “Distributed fluorescence sensing using exposed-core microstructured optical fiber,” IEEE Photon. Technol. Lett.22(18), 1385–1387 (2010). [CrossRef]
  14. E. Coscelli, M. Sozzi, F. Poli, D. Passaro, A. Cucinotta, S. Selleri, R. Corradini, and R. Marchelli, “Toward A Highly Specific DNA Biosensor: PNA-Modified Suspended-Core Photonic Crystal Fibers,” IEEE Sel. Top. Quantum. Electron.16(4), 967–972 (2010). [CrossRef]
  15. S. Tyagi and F. R. Kramer, “Molecular Beacons: Probes that Fluoresce upon Hybridization,” Nat. Biotechnol.14(3), 303–308 (1996). [CrossRef] [PubMed]
  16. http://www.molecular-beacons.com/MB_publications.html
  17. X. Liu and W. Tan, “A Fiber-Optic Evanescent Wave DNA Biosensor Based on Novel Molecular Beacons,” Anal. Chem.71(22), 5054–5059 (1999). [CrossRef] [PubMed]
  18. Y. Sun, and X. Fan, “Highly Selective Single-Nucleotide Polymorphism Detection with Optofluidic Ring Resonator Lasers,” in CLEO - Laser Applications to Photonic Applications, Technical Digest (CD) (Optical Society of America, 2011), paper CWL6.
  19. G. Decher, “Fuzzy Nanoassemblies: Toward layered polymeric multicomposites,” Science277(5330), 1232–1237 (1997). [CrossRef]
  20. M. Grabka, S. Pustelny, P. Mergo, and W. Gawlik, “Application of continuous wavelet transform for determination of fiber birefringence,” Opt. Express20(13), 13878–13885 (2012). [CrossRef] [PubMed]
  21. X. Liu, W. Farmerie, S. Schuster, and W. Tan, “Molecular beacons for DNA biosensors with Micrometer to Submicrometer dimensions,” Anal. Biochem.283(1), 56–63 (2000). [CrossRef] [PubMed]
  22. D. G. Wang, J. B. Fan, C. J. Siao, A. Berno, P. Young, R. Sapolsky, G. Ghandour, N. Perkins, E. Winchester, J. Spencer, L. Kruglyak, L. Stein, L. Hsie, T. Topaloglou, E. Hubbell, E. Robinson, M. Mittmann, M. S. Morris, N. Shen, D. Kilburn, J. Rioux, C. Nusbaum, S. Rozen, T. J. Hudson, R. Lipshutz, M. Chee, and E. S. Lander, “Large-scale Identification, Mapping, and Genotyping of Single-nucleotide polymorphisms in the human genome,” Science280(5366), 1077–1082 (1998). [CrossRef] [PubMed]

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