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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 1 — Jan. 14, 2013
  • pp: 112–121

Super-resolution differential interference contrast microscopy by structured illumination

Jianling Chen, Yan Xu, Xiaohua Lv, Xiaomin Lai, and Shaoqun Zeng  »View Author Affiliations


Optics Express, Vol. 21, Issue 1, pp. 112-121 (2013)
http://dx.doi.org/10.1364/OE.21.000112


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Abstract

We propose a structured illumination differential interference contrast (SI-DIC) microscopy, breaking the diffraction resolution limit of differential interference contrast (DIC) microscopy. SI-DIC extends the bandwidth of coherent transfer function of the DIC imaging system, thus the resolution is improved. With 0.8 numerical aperture condenser and objective, the reconstructed SI-DIC image of 53 nm polystyrene beads reveals lateral resolution of approximately 190 nm, doubling that of the conventional DIC image. We also demonstrate biological observations of label-free cells with improved spatial resolution. The SI-DIC microscopy can provide sub-diffraction resolution and high contrast images with marker-free specimens, and has the potential for achieving sub-diffraction resolution quantitative phase imaging.

© 2013 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(110.1650) Imaging systems : Coherence imaging
(180.0180) Microscopy : Microscopy

ToC Category:
Microscopy

History
Original Manuscript: October 23, 2012
Revised Manuscript: December 17, 2012
Manuscript Accepted: December 17, 2012
Published: January 2, 2013

Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Jianling Chen, Yan Xu, Xiaohua Lv, Xiaomin Lai, and Shaoqun Zeng, "Super-resolution differential interference contrast microscopy by structured illumination," Opt. Express 21, 112-121 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-1-112


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References

  1. S. Bradbury and P. Evennett, Contrast Techniques in Light Microscopy (BIOS Scientific, 1996).
  2. R. A. Hoebe, C. H. Van Oven, T. W. J. Gadella, P. B. Dhonukshe, C. J. Van Noorden, and E. M. Manders, “Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging,” Nat. Biotechnol.25(2), 249–253 (2007). [CrossRef] [PubMed]
  3. H. Gundlach, “Phase contrast and differential interference contrast instrumentation and applications in cell, developmental, and marine biology,” Opt. Eng.32(12), 3223–3228 (1993). [CrossRef]
  4. H. Ooki, Y. Iwasaki, and J. Iwasaki, “Differential interference contrast microscope with differential detection for optimizing image contrast,” Appl. Opt.35(13), 2230–2234 (1996). [CrossRef] [PubMed]
  5. C. Preza, D. L. Snyder, and J. A. Conchello, “Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy,” J. Opt. Soc. Am. A16(9), 2185–2199 (1999). [CrossRef] [PubMed]
  6. T. J. McIntyre, C. Maurer, S. Bernet, and M. Ritsch-Marte, “Differential interference contrast imaging using a spatial light modulator,” Opt. Lett.34(19), 2988–2990 (2009). [CrossRef] [PubMed]
  7. T. Kim, S. Sridharan, and G. Popescu, “Gradient field microscopy of unstained specimens,” Opt. Express20(6), 6737–6745 (2012). [CrossRef] [PubMed]
  8. F. Zernike, “Diffraction theory of the knife-edge test and its improved form, the phase-contrast method,” Mon. Not. R. Astron. Soc.94, 377–384 (1934).
  9. D. Fu, S. Oh, W. Choi, T. Yamauchi, A. Dorn, Z. Yaqoob, R. R. Dasari, and M. S. Feld, “Quantitative DIC microscopy using an off-axis self-interference approach,” Opt. Lett.35(14), 2370–2372 (2010). [CrossRef] [PubMed]
  10. R. D. Allen, N. S. Allen, and J. L. Travis, “Video-enhanced contrast, differential interference contrast (AVEC-DIC) microscopy: A new method capable of analyzing microtubule-related motility in the reticulopodial network of allogromia laticollaris,” Cell Motil.1(3), 291–302 (1981). [CrossRef] [PubMed]
  11. G. M. Langford, “Video-enhanced microscopy for analysis of cytoskeleton structure and function,” Methods Mol. Biol.161, 31–43 (2001). [PubMed]
  12. S. W. Hell and J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy,” Opt. Lett.19(11), 780–782 (1994). [CrossRef] [PubMed]
  13. M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc.198(2), 82–87 (2000). [CrossRef] [PubMed]
  14. M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006). [CrossRef] [PubMed]
  15. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006). [CrossRef] [PubMed]
  16. B. Littleton, K. Lai, D. Longstaff, V. Sarafis, P. Munroe, N. Heckenberg, and H. Rubinsztein-Dunlop, “Coherent super-resolution microscopy via laterally structured illumination,” Micron38(2), 150–157 (2007). [CrossRef] [PubMed]
  17. S. A. Shroff, “Structured Illumination Imaging,” Ph.D thesis (The University of Rochester, 2010).
  18. B. J. Chang, S. H. Lin, L. J. Chou, and S. Y. Chiang, “Subdiffraction scattered light imaging of gold nanoparticles using structured illumination,” Opt. Lett.36(24), 4773–4775 (2011). [CrossRef] [PubMed]
  19. S. Chowdhury, A. H. Dhalla, and J. Izatt, “Structured oblique illumination microscopy for enhanced resolution imaging of non-fluorescent, coherently scattering samples,” Biomed. Opt. Express3(8), 1841–1854 (2012). [CrossRef] [PubMed]
  20. P. Hariharan, “The Sénarmont Compensator: An Early Application of the Geometric Phase,” J. Mod. Opt.40(11), 2061–2064 (1993). [CrossRef]
  21. J. W. Goodman, Introduction to Fourier optics 2nd Edition (McGraw Hill, 1996).
  22. S. B. Mehta and C. J. Sheppard, “Partially coherent image formation in differential interference contrast (DIC) microscope,” Opt. Express16(24), 19462–19479 (2008). [CrossRef] [PubMed]
  23. B. J. Chang, L. J. Chou, Y. C. Chang, and S. Y. Chiang, “Isotropic image in structured illumination microscopy patterned with a spatial light modulator,” Opt. Express17(17), 14710–14721 (2009). [CrossRef] [PubMed]
  24. S. V. King, “Quantitative phase information from differential interference contrast microscopy,” Ph. D thesis (University of Colorado at Boulder, 2009).
  25. S. D. Babacan, Z. Wang, M. Do, and G. Popescu, “Cell imaging beyond the diffraction limit using sparse deconvolution spatial light interference microscopy,” Biomed. Opt. Express2(7), 1815–1827 (2011). [CrossRef] [PubMed]
  26. C. J. Cogswell, N. I. Smith, K. G. Larkin, and P. Hariharan, “Quantitative DIC microscopy using a geometric phase shifter,” Proc. SPIE2984, 72–81 (1997). [CrossRef]
  27. T. J. McIntyre, C. Maurer, S. Fassl, S. Khan, S. Bernet, and M. Ritsch-Marte, “Quantitative SLM-based differential interference contrast imaging,” Opt. Express18(13), 14063–14078 (2010). [CrossRef] [PubMed]
  28. Y. Wu, X. Liu, W. Zhou, X. Lv, and S. Zeng, “Observing neuronal activities with random access two-photon microscope,” J. Innov. Opt. Health Sci.02(01), 67–71 (2009). [CrossRef]
  29. M. A. Komandirov, E. A. Knyazeva, Y. P. Fedorenko, M. V. Rudkovskii, E. V. Berezhnaya, V. D. Kovaleva, and A. B. Uzdensky, “Chemical modulation of photodynamic injury of glial cells,” J. Innov. Opt. Health Sci.04(04), 429–435 (2011). [CrossRef]

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