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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 10 — May. 20, 2013
  • pp: 12228–12237

Accurate holographic imaging of colloidal particle pairs by Rayleigh-Sommerfeld reconstruction

David Kapfenberger, Adar Sonn-Segev, and Yael Roichman  »View Author Affiliations

Optics Express, Vol. 21, Issue 10, pp. 12228-12237 (2013)

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In-line holographic optical imaging has the unique capability of high speed imaging in three dimensions at rates limited only by the imaging rate of the camera used. In this technique the 3D data is recorded on the detector in a form of a hologram generated by diffraction between the scattered and unscattered light passing through the sample. For dilute samples of single particles or a small cluster of particles, this technique was shown to result in particle tracking with spatial positioning accuracy of a few nanometers. For dense suspension only approximate reconstruction were achieved with systematic axial positioning errors. We propose a scheme to extend accurate holographic microscopy to dense suspensions, by calibrating the Rayleigh-Sommerfeld reconstruction algorithm against Lorentz-Mie scattering theory. We perform this calibration both numerically and experimentally and define the parameter space in which accurate imaging is achieved, and in which numerical calibration holds. We demonstrate the validity of our approach by imaging two attached particles and measuring the distance between their centers with 36 nm accuracy. A difference of 50 nm in particle diameter is easily measured.

© 2013 OSA

OCIS Codes
(090.1760) Holography : Computer holography
(100.3010) Image processing : Image reconstruction techniques
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: December 18, 2012
Revised Manuscript: March 1, 2013
Manuscript Accepted: March 5, 2013
Published: May 10, 2013

Virtual Issues
Vol. 8, Iss. 6 Virtual Journal for Biomedical Optics

David Kapfenberger, Adar Sonn-Segev, and Yael Roichman, "Accurate holographic imaging of colloidal particle pairs by Rayleigh-Sommerfeld reconstruction," Opt. Express 21, 12228-12237 (2013)

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