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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 13 — Jul. 1, 2013
  • pp: 15475–15489

Quantitative fluorescence and elastic scattering tissue polarimetry using an Eigenvalue calibrated spectroscopic Mueller matrix system

Jalpa Soni, Harsh Purwar, Harshit Lakhotia, Shubham Chandel, Chitram Banerjee, Uday Kumar, and Nirmalya Ghosh  »View Author Affiliations

Optics Express, Vol. 21, Issue 13, pp. 15475-15489 (2013)

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A novel spectroscopic Mueller matrix system has been developed and explored for both fluorescence and elastic scattering polarimetric measurements from biological tissues. The 4 × 4 Mueller matrix measurement strategy is based on sixteen spectrally resolved (λ = 400 - 800 nm) measurements performed by sequentially generating and analyzing four elliptical polarization states. Eigenvalue calibration of the system ensured high accuracy of Mueller matrix measurement over a broad wavelength range, either for forward or backscattering geometry. The system was explored for quantitative fluorescence and elastic scattering spectroscopic polarimetric studies on normal and precancerous tissue sections from human uterine cervix. The fluorescence spectroscopic Mueller matrices yielded an interesting diattenuation parameter, exhibiting differences between normal and precancerous tissues.

© 2013 OSA

OCIS Codes
(120.5410) Instrumentation, measurement, and metrology : Polarimetry
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.7050) Medical optics and biotechnology : Turbid media
(260.5430) Physical optics : Polarization
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
(170.6935) Medical optics and biotechnology : Tissue characterization
(240.2130) Optics at surfaces : Ellipsometry and polarimetry

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 25, 2013
Revised Manuscript: May 13, 2013
Manuscript Accepted: May 13, 2013
Published: June 21, 2013

Virtual Issues
Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

Jalpa Soni, Harsh Purwar, Harshit Lakhotia, Shubham Chandel, Chitram Banerjee, Uday Kumar, and Nirmalya Ghosh, "Quantitative fluorescence and elastic scattering tissue polarimetry using an Eigenvalue calibrated spectroscopic Mueller matrix system," Opt. Express 21, 15475-15489 (2013)

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