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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 13 — Jul. 1, 2013
  • pp: 16007–16021

Instantaneous three-dimensional sensing using spatial light modulator illumination with extended depth of field imaging

Sean Quirin, Darcy S. Peterka, and Rafael Yuste  »View Author Affiliations

Optics Express, Vol. 21, Issue 13, pp. 16007-16021 (2013)

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Imaging three-dimensional structures represents a major challenge for conventional microscopies. Here we describe a Spatial Light Modulator (SLM) microscope that can simultaneously address and image multiple targets in three dimensions. A wavefront coding element and computational image processing enables extended depth-of-field imaging. High-resolution, multi-site three-dimensional targeting and sensing is demonstrated in both transparent and scattering media over a depth range of 300-1,000 microns.

© 2013 OSA

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(110.1758) Imaging systems : Computational imaging
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Imaging Systems

Original Manuscript: April 26, 2013
Revised Manuscript: June 12, 2013
Manuscript Accepted: June 13, 2013
Published: June 27, 2013

Virtual Issues
Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

Sean Quirin, Darcy S. Peterka, and Rafael Yuste, "Instantaneous three-dimensional sensing using spatial light modulator illumination with extended depth of field imaging," Opt. Express 21, 16007-16021 (2013)

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