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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 17 — Aug. 26, 2013
  • pp: 19701–19708

4Pi fluorescence detection and 3D particle localization with a single objective

J. Schnitzbauer, R. McGorty, and B. Huang  »View Author Affiliations


Optics Express, Vol. 21, Issue 17, pp. 19701-19708 (2013)
http://dx.doi.org/10.1364/OE.21.019701


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Abstract

Coherent detection through two opposing objectives (4Pi configuration) improves the precision of three-dimensional (3D) single-molecule localization substantially along the axial direction, but suffers from instrument complexity and maintenance difficulty. To address these issues, we have realized 4Pi fluorescence detection by sandwiching the sample between the objective and a mirror, and create interference of direct incidence and mirror-reflected signal at the camera with a spatial light modulator. Multifocal imaging using this single-objective mirror interference scheme offers improvement in the axial localization similar to the traditional 4Pi method. We have also devised several PSF engineering schemes to enable 3D localization with a single emitter image, offering better axial precision than normal single-objective localization methods such as astigmatic imaging.

© 2013 OSA

OCIS Codes
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.2520) Microscopy : Fluorescence microscopy
(180.3170) Microscopy : Interference microscopy
(350.5730) Other areas of optics : Resolution
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Microscopy

History
Original Manuscript: June 21, 2013
Revised Manuscript: August 6, 2013
Manuscript Accepted: August 7, 2013
Published: August 14, 2013

Virtual Issues
Vol. 8, Iss. 9 Virtual Journal for Biomedical Optics

Citation
J. Schnitzbauer, R. McGorty, and B. Huang, "4Pi fluorescence detection and 3D particle localization with a single objective," Opt. Express 21, 19701-19708 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-17-19701


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References

  1. G. J. Schütz, V. Ph. Pastushenko, H. J. Gruber, H.-G. Knaus, B. Pragl, and H. Schindler, “3D Imaging of Individual Ion Channels in Live Cells at 40nm Resolution,” Single Molecules1(1), 25–31 (2000). [CrossRef]
  2. M. Speidel, A. Jonás, and E. L. Florin, “Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging,” Opt. Lett.28(2), 69–71 (2003). [CrossRef] [PubMed]
  3. T. M. Watanabe, T. Sato, K. Gonda, and H. Higuchi, “Three-dimensional nanometry of vesicle transport in living cells using dual-focus imaging optics,” Biochem. Biophys. Res. Commun.359(1), 1–7 (2007). [CrossRef] [PubMed]
  4. E. Toprak, H. Balci, B. H. Blehm, and P. R. Selvin, “Three-dimensional particle tracking via bifocal imaging,” Nano Lett.7(7), 2043–2045 (2007). [CrossRef] [PubMed]
  5. M. F. Juette and J. Bewersdorf, “Three-dimensional tracking of single fluorescent particles with submillisecond temporal resolution,” Nano Lett.10(11), 4657–4663 (2010). [CrossRef] [PubMed]
  6. S. Ram, P. Prabhat, J. Chao, E. S. Ward, and R. J. Ober, “High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells,” Biophys. J.95(12), 6025–6043 (2008). [CrossRef] [PubMed]
  7. S. Ram, P. Prabhat, E. S. Ward, and R. J. Ober, “Improved single particle localization accuracy with dual objective multifocal plane microscopy,” Opt. Express17(8), 6881–6898 (2009). [CrossRef] [PubMed]
  8. H. P. Kao and A. S. Verkman, “Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position,” Biophys. J.67(3), 1291–1300 (1994). [CrossRef] [PubMed]
  9. L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett.90(5), 053902 (2007). [CrossRef]
  10. B. Huang, W. Wang, M. Bates, and X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science319(5864), 810–813 (2008). [CrossRef] [PubMed]
  11. S. R. Pavani and R. Piestun, “Three dimensional tracking of fluorescent microparticles using a photon-limited double-helix response system,” Opt. Express16(26), 22048–22057 (2008). [CrossRef] [PubMed]
  12. S. R. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.106(9), 2995–2999 (2009). [CrossRef] [PubMed]
  13. S. R. Pavani, J. G. DeLuca, and R. Piestun, “Polarization sensitive, three-dimensional, single-molecule imaging of cells with a double-helix system,” Opt. Express17(22), 19644–19655 (2009). [CrossRef] [PubMed]
  14. G. Grover, S. Quirin, C. Fiedler, and R. Piestun, “Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging,” Biomed. Opt. Express2(11), 3010–3020 (2011). [CrossRef] [PubMed]
  15. S. Quirin, S. R. Pavani, and R. Piestun, “Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions,” Proc. Natl. Acad. Sci. U.S.A.109(3), 675–679 (2012). [CrossRef] [PubMed]
  16. G. Grover, K. DeLuca, S. Quirin, J. DeLuca, and R. Piestun, “Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE),” Opt. Express20(24), 26681–26695 (2012). [CrossRef] [PubMed]
  17. M. A. Thompson, J. M. Casolari, M. Badieirostami, P. O. Brown, and W. E. Moerner, “Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A.107(42), 17864–17871 (2010). [CrossRef] [PubMed]
  18. M. D. Lew, S. F. Lee, M. Badieirostami, and W. E. Moerner, “Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects,” Opt. Lett.36(2), 202–204 (2011). [CrossRef] [PubMed]
  19. C. von Middendorff, A. Egner, C. Geisler, S. W. Hell, and A. Schönle, “Isotropic 3D Nanoscopy based on single emitter switching,” Opt. Express16(25), 20774–20788 (2008). [CrossRef] [PubMed]
  20. M. J. Mlodzianoski, M. F. Juette, G. L. Beane, and J. Bewersdorf, “Experimental characterization of 3D localization techniques for particle-tracking and super-resolution microscopy,” Opt. Express17(10), 8264–8277 (2009). [CrossRef] [PubMed]
  21. M. Badieirostami, M. D. Lew, M. A. Thompson, and W. E. Moerner, “Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane,” Appl. Phys. Lett.97(16), 161103 (2010). [CrossRef] [PubMed]
  22. G. Grover, S. R. Pavani, and R. Piestun, “Performance limits on three-dimensional particle localization in photon-limited microscopy,” Opt. Lett.35(19), 3306–3308 (2010). [CrossRef] [PubMed]
  23. G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” Proc. Natl. Acad. Sci. U.S.A.106(9), 3125–3130 (2009). [CrossRef] [PubMed]
  24. D. Aquino, A. Schönle, C. Geisler, C. V. Middendorff, C. A. Wurm, Y. Okamura, T. Lang, S. W. Hell, and A. Egner, “Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores,” Nat. Methods8(4), 353–359 (2011). [CrossRef] [PubMed]
  25. J. Bewersdorf, A. Egner, and S. W. Hell, “4Pi Microscopy,” in Handbook of Biological Confocal Microscopy, J. Pawley, ed. (Springer, New York, 2006), pp. 561–570.
  26. M. G. Gustafsson, D. A. Agard, and J. W. Sedat, “I5M: 3D widefield light microscopy with better than 100 nm axial resolution,” J. Microsc.195(1), 10–16 (1999). [CrossRef] [PubMed]
  27. A. Egner and S. W. Hell, “Fluorescence microscopy with super-resolved optical sections,” Trends Cell Biol.15(4), 207–215 (2005). [CrossRef] [PubMed]
  28. L. Moiseev, C. R. Cantor, M. I. Aksun, M. Dogan, B. B. Goldberg, A. K. Swan, and M. S. Unlu, “Spectral self-interference fluorescence microscopy,” J. Appl. Phys.96(9), 5311–5315 (2004). [CrossRef]
  29. L. Moiseev, M. S. Unlü, A. K. Swan, B. B. Goldberg, and C. R. Cantor, “DNA conformation on surfaces measured by fluorescence self-interference,” Proc. Natl. Acad. Sci. U.S.A.103(8), 2623–2628 (2006). [CrossRef] [PubMed]
  30. M. J. Paszek, C. C. DuFort, M. G. Rubashkin, M. W. Davidson, K. S. Thorn, J. T. Liphardt, and V. M. Weaver, “Scanning angle interference microscopy reveals cell dynamics at the nanoscale,” Nat. Methods9(8), 825–827 (2012). [CrossRef] [PubMed]
  31. S. Zwick, T. Haist, Y. Miyamoto, L. He, M. Warber, A. Hermerschmidt, and W. Osten, “Holographic twin traps,” J Opt a-Pure Appl Op 11 (2009).
  32. M. Pitzek, R. Steiger, G. Thalhammer, S. Bernet, and M. Ritsch-Marte, “Optical mirror trap with a large field of view,” Opt. Express17(22), 19414–19423 (2009). [CrossRef] [PubMed]
  33. E. Mudry, E. Le Moal, P. Ferrand, P. C. Chaumet, and A. Sentenac, “Isotropic diffraction-limited focusing using a single objective lens,” Phys. Rev. Lett.105(20), 203903 (2010). [CrossRef] [PubMed]
  34. E. Le Moal, E. Mudry, P. C. Chaumet, P. Ferrand, and A. Sentenac, “Isotropic single-objective microscopy: theory and experiment,” J. Opt. Soc. Am. A28(8), 1586–1594 (2011). [CrossRef] [PubMed]
  35. S. Li, C. F. Kuang, X. Hao, Z. Gu, and X. T. Liu, “Generation of a 3D isotropic hollow focal spot for single-objective stimulated emission depletion microscopy,” J Optics-Uk 14 (2012).
  36. B. M. Hanser, M. G. Gustafsson, D. A. Agard, and J. W. Sedat, “Phase-retrieved pupil functions in wide-field fluorescence microscopy,” J. Microsc.216(1), 32–48 (2004). [CrossRef] [PubMed]
  37. R. J. Ober, S. Ram, and E. S. Ward, “Localization accuracy in single-molecule microscopy,” Biophys. J.86(2), 1185–1200 (2004). [CrossRef] [PubMed]
  38. K. I. Mortensen, L. S. Churchman, J. A. Spudich, and H. Flyvbjerg, “Optimized localization analysis for single-molecule tracking and super-resolution microscopy,” Nat. Methods7(5), 377–381 (2010). [CrossRef] [PubMed]
  39. C. S. Smith, N. Joseph, B. Rieger, and K. A. Lidke, “Fast, single-molecule localization that achieves theoretically minimum uncertainty,” Nat. Methods7(5), 373–375 (2010). [CrossRef] [PubMed]
  40. R. W. Deming, “Phase retrieval from intensity-only data by relative entropy minimization,” J. Opt. Soc. Am. A24(11), 3666–3679 (2007). [CrossRef] [PubMed]
  41. P. Kner, L. Winoto, D. A. Agard, and J. W. Sedat, “Closed loop adaptive optics for microscopy without a wavefront sensor,” Proc. SPIE7570(757006), 757006 (2010).
  42. R. Di Leonardo, F. Ianni, and G. Ruocco, “Computer generation of optimal holograms for optical trap arrays,” Opt. Express15(4), 1913–1922 (2007). [CrossRef] [PubMed]
  43. K. Rastani, A. Marrakchi, S. F. Habiby, W. M. Hubbard, H. Gilchrist, and R. E. Nahory, “Binary Phase Fresnel Lenses for Generation of two-Dimensional Beam Arrays,” Appl. Opt.30(11), 1347–1354 (1991). [CrossRef] [PubMed]

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