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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 19 — Sep. 23, 2013
  • pp: 22285–22313

Fluorescence of bioaerosols: mathematical model including primary fluorescing and absorbing molecules in bacteria

Steven C. Hill, Yong-Le Pan, Chatt Williamson, Joshua L. Santarpia, and Hanna H. Hill  »View Author Affiliations

Optics Express, Vol. 21, Issue 19, pp. 22285-22313 (2013)

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This paper describes a mathematical model of fluorescent biological particles composed of bacteria, viruses, or proteins. The fluorescent and/or light absorbing molecules included in the model are amino acids (tryptophan, etc.); nucleic acids (DNA, RNA, etc.); coenzymes (nicotinamide adenine dinucleotides, flavins, and vitamins B6 and K and variants of these); and dipicolinates. The concentrations, absorptivities, and fluorescence quantum yields are estimated from the literature, often with large uncertainties. The bioparticles in the model are spherical and homogeneous. Calculated fluorescence cross sections for particles excited at 266, 280, and 355 nm are compared with measured values from the literature for several bacteria, bacterial spores and albumins. The calculated 266- and 280-nm excited fluorescence is within a factor of 3.2 of the measurements for the vegetative cells and proteins, but overestimates the fluorescence of spores by a factor of 10 or more. This is the first reported modeling of the fluorescence of bioaerosols in which the primary fluorophores and absorbing molecules are included.

© 2013 OSA

OCIS Codes
(010.1100) Atmospheric and oceanic optics : Aerosol detection
(160.2540) Materials : Fluorescent and luminescent materials
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(290.1090) Scattering : Aerosol and cloud effects
(290.5850) Scattering : Scattering, particles
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Atmospheric and Oceanic Optics

Original Manuscript: May 30, 2013
Revised Manuscript: July 19, 2013
Manuscript Accepted: July 20, 2013
Published: September 13, 2013

Virtual Issues
Vol. 8, Iss. 10 Virtual Journal for Biomedical Optics

Steven C. Hill, Yong-Le Pan, Chatt Williamson, Joshua L. Santarpia, and Hanna H. Hill, "Fluorescence of bioaerosols: mathematical model including primary fluorescing and absorbing molecules in bacteria," Opt. Express 21, 22285-22313 (2013)

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