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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 21 — Oct. 21, 2013
  • pp: 25221–25235

Measuring the scattering coefficient of turbid media from two-photon microscopy

David Sevrain, Matthieu Dubreuil, Aymeric Leray, Christophe Odin, and Yann Le Grand  »View Author Affiliations

Optics Express, Vol. 21, Issue 21, pp. 25221-25235 (2013)

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In this paper, we propose a new and simple method based on two-photon excitation fluorescence (TPEF) microscopy to measure the scattering coefficient µs of thick turbid media. We show, from Monte Carlo simulations, that µs can be derived from the axial profile of the ratio of the TPEF signals epi-collected by the confocal and the non-descanned ports of a scanning microscope, independently of the anisotropy factor g and of the absorption coefficient µa of the medium. The method is validated experimentally on tissue-mimicking optical phantoms, and is shown to have potential for imaging the scattering coefficient of heterogeneous media.

© 2013 OSA

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(180.1790) Microscopy : Confocal microscopy
(290.5820) Scattering : Scattering measurements
(290.7050) Scattering : Turbid media
(180.4315) Microscopy : Nonlinear microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 18, 2013
Revised Manuscript: July 11, 2013
Manuscript Accepted: July 12, 2013
Published: October 15, 2013

David Sevrain, Matthieu Dubreuil, Aymeric Leray, Christophe Odin, and Yann Le Grand, "Measuring the scattering coefficient of turbid media from two-photon microscopy," Opt. Express 21, 25221-25235 (2013)

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