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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 22 — Nov. 4, 2013
  • pp: 25655–25663

High-resolution microscopy for biological specimens via cathodoluminescence of Eu- and Zn-doped Y2O3nanophosphors

Taichi Furukawa, Hirohiko Niioka, Masayoshi Ichimiya, Tomohiro Nagata, Masaaki Ashida, Tsutomu Araki, and Mamoru Hashimoto  »View Author Affiliations


Optics Express, Vol. 21, Issue 22, pp. 25655-25663 (2013)
http://dx.doi.org/10.1364/OE.21.025655


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Abstract

High-resolution microscopy for biological specimens was performed using cathodoluminescence (CL) of Y2O3:Eu, Zn nanophosphors, which have high CL intensity due to the incorporation of Zn. The intensity of Y2O3:Eu nanophosphors at low acceleration voltage (3 kV) was increased by adding Zn. The CL intensity was high enough for imaging even with a phosphor size as small as about 30 nm. The results show the possibility of using CL microscopy for biological specimens at single-protein-scale resolution. CL imaging of HeLa cells containing laser-ablated Y2O3:Eu, Zn nanophosphors achieved a spatial resolution of a few tens of nanometers. Y2O3:Eu, Zn nanophosphors in HeLa cells were also imaged with 254 nm ultraviolet light excitation. The results suggest that correlative microscopy using CL, secondary electrons and fluorescence imaging could enable multi-scale investigation of molecular localization from the nanoscale to the microscale.

© 2013 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Microscopy

History
Original Manuscript: June 21, 2013
Revised Manuscript: August 6, 2013
Manuscript Accepted: September 9, 2013
Published: October 21, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Taichi Furukawa, Hirohiko Niioka, Masayoshi Ichimiya, Tomohiro Nagata, Masaaki Ashida, Tsutomu Araki, and Mamoru Hashimoto, "High-resolution microscopy for biological specimens via cathodoluminescence of Eu- and Zn-doped Y2O3nanophosphors," Opt. Express 21, 25655-25663 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-22-25655


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References

  1. J. W. Stirling, “Immuno- and affinity probes for electron microscopy: a review of labeling and preparation techniques,” J. Histochem. Cytochem.38(2), 145–157(1990). [CrossRef] [PubMed]
  2. M. C. Risueño and P. S. Testillano, “Cytochemistry and immunocytochemistry of nucleolar chromatin in plants,” Micron25(4), 331–360(1994). [CrossRef] [PubMed]
  3. B. L. Wang and L. I. Larsson, “Simultaneous demonstration of multiple antigens by indirect immunofluorescence or immunogold staining. Novel light and electron microscopical double and triple staining method employing primary antibodies from the same species,” Histochemistry83(1), 47–56(1985). [CrossRef] [PubMed]
  4. R. S. Petralia, J. A. Esteban, Y. X. Wang, J. G. Partridge, H. M. Zhao, R. J. Wenthold, and R. Malinow, “Selective acquisition of AMPA receptors over postnatal development suggests a molecular basis for silent synapses,” Nat. Neurosci.2(1), 31–36(1999). [CrossRef] [PubMed]
  5. H. J. Geuze, J. W. Slot, P. A. van der Ley, R. C. Scheffer, and J. M. Griffith, “Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections,” J. Cell Biol.89(3), 653–665(1981). [CrossRef] [PubMed]
  6. B. Huang, M. Bates, and X. Zhuang, “Super-resolution fluorescence microscopy,” Annu. Rev. Biochem.78(1), 993–1016(2009). [CrossRef] [PubMed]
  7. B. O. Leung and K. C. Chou, “Review of super-resolution fluorescence microscopy for biology,” Appl. Spectrosc.65(9), 967–980(2011). [CrossRef] [PubMed]
  8. T. M. Svitkina and G. G. Borisy, “Correlative light and electron microscopy of the cytoskeleton of cultured cells,” Methods Enzymol.298, 570–592(1998). [CrossRef] [PubMed]
  9. T. M. Svitkina, E. A. Bulanova, O. Y. Chaga, D. M. Vignjevic, S. Kojima, J. M. Vasiliev, and G. G. Borisy, “Mechanism of filopodia initiation by reorganization of a dendritic network,” J. Cell Biol.160(3), 409–421(2003). [CrossRef] [PubMed]
  10. B. G. Yacobi and D. B. Holt, Cathodoluminescence Microscopy of Inorganic Solids(Plenum, 1990).
  11. H. Niioka, T. Furukawa, M. Ichimiya, M. Ashida, T. Araki, and M. Hashimoto, “Multicolor cathodoluminescence microscopy for biological imaging with nanophosphors,” Appl. Phys. Express4(11), 112402(2011). [CrossRef]
  12. P. J. Fisher, W. S. Wessels, A. B. Dietz, and F. G. Prendergast, “Enhanced biological cathodoluminescence,” Opt. Commun.281(7), 1901–1908(2008). [CrossRef]
  13. Y. Nawa, W. Inami, A. Chiba, A. Ono, A. Miyakawa, Y. Kawata, S. Lin, and S. Terakawa, “Dynamic and high-resolution live cell imaging by direct electron beam excitation,” Opt. Express20(5), 5629–5635(2012). [CrossRef] [PubMed]
  14. J. Niitsuma, H. Oikawa, E. Kimura, T. Ushiki, and T. Sekiguchi, “Cathodoluminescence investigation of organic materials,” J. Electron Microsc. (Tokyo)54(4), 325–330(2005). [CrossRef] [PubMed]
  15. P. J. Fisher, W. S. Wessels, A. B. Dietz, and F. G. Prendergast, “Immuno-fluorescence scanning electron microscopy of biological cells,” Opt. Commun.18(5), 8–13(2010).
  16. W. M. Yen, S. Shionoya, and H. Yamamoto, Phosphor Handbook(CRC, 2007).
  17. J. Hao, S. A. Studenikin, and M. J. Cocivera, “Blue, green and red cathodoluminescence of Y2O3phosphor films prepared by spray pyrolysis,” Lumin.93(4), 313–319(2001). [CrossRef]
  18. T. Nagata, T. Nakayama, and H. Murakami, “Low voltage cathodoluminescent properties of Y2O3-based nano-particle phosphors,” SID’ 07 Digest(2007), 1328-1331(2007).
  19. S. Sakuma, H. Kominami, Y. Neo, T. Aoki, Y. Nakanishi, and H. Mimura, “Effect of La and Zn addition on Y2O3:Eu phosphors,” Appl. Surf. Sci.244(1-4), 458–460(2005). [CrossRef]
  20. F. Mafuné, J. Kohno, Y. Takeda, T. Kondow, and H. Sawabe, “Formation and size control of silver nanoparticles by laser ablation in aqueous solution,” J. Phys. Chem. B104(39), 9111–9117(2000). [CrossRef]
  21. A. Murali, A. Barve, V. J. Leppert, S. H. Risbud, I. M. Kennedy, and H. W. H. Lee, “Synthesis and characterization of indium oxide nanoparticles,” Nano Lett.1(6), 287–289(2001). [CrossRef]
  22. G. Ledoux, D. Amans, C. Dujardin, and K. Masenelli-Varlot, “Facile and rapid synthesis of highly luminescent nanoparticles via pulsed laser ablation in liquid,” Nanotechnology20(44), 445605(2009). [CrossRef] [PubMed]
  23. H. Zeng, S. Yang, and W. Cai, “Reshaping formation and luminescence evolution of ZnO quantum dots by laser-induced fragmentation in liquid,” J. Phys. Chem. C115(12), 5038–5043(2011). [CrossRef]
  24. H. Niioka, N. I. Smith, K. Fujita, Y. Inouye, and S. Kawata, “Femtosecond laser nano-ablation in fixed and non-fixed cultured cells,” Opt. Express16(19), 14476–14495(2008). [CrossRef] [PubMed]
  25. D. C. Joy, Monte Carlo Modeling for Electron Microscopy and Microanalysis(Oxford, 1995).
  26. N. Yamamoto, K. Araya, and F. García de Abajo, “Photon emission from silver particles induced by a high-energy electron beam,” J. Phys. Rev. B64(20), 205419(2001). [CrossRef]
  27. N. Yamamoto, S. Ohtani, and F. J. García de Abajo, “Gap and Mie plasmons in individual silver nanospheres near a silver surface,” Nano Lett.11(1), 91–95(2011). [CrossRef] [PubMed]
  28. S. Chandra, F. L. Deepak, J. B. Gruber, and D. K. Sardar, “Synthesis, morphology, and optical characterization of nanocrystalline Er3+:Y2O3,” J. Phys. Chem. C114(2), 874–880(2010). [CrossRef]
  29. V. Singh, V. K. Rai, I. Ledoux-Rak, S. Watanabe, T. K. G. Rao, J. F. D. Chucaci, L. Badie, F. Pelle, and S. Ivanova, “NIR to visible up-conversion, infrared luminescence, thermoluminescence and defect centres in Y2O3: Er phosphor,” J. Phys. D Appl. Phys.42(6), 065104(2009). [CrossRef]
  30. S. F. Lim, R. Riehn, W. S. Ryu, N. Khanarian, C. K. Tung, D. Tank, and R. H. Austin, “In vivo and scanning electron microscopy imaging of up-converting nanophosphors in Caenorhabditis elegans,” Nano Lett.6(2), 169–174(2006). [CrossRef] [PubMed]
  31. S. Thiberge, A. Nechushtan, D. Sprinzak, O. Gileadi, V. Behar, O. Zik, Y. Chowers, S. Michaeli, J. Schlessinger, and E. Moses, “Scanning electron microscopy of cells and tissues under fully hydrated conditions,” Proc. Natl. Acad. Sci. U.S.A.101(10), 3346–3351(2004). [CrossRef] [PubMed]
  32. N. de Jonge and F. M. Ross, “Electron microscopy of specimens in liquid,” Nat. Nanotechnol.6(11), 695–704(2011). [CrossRef] [PubMed]

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