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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 22 — Nov. 4, 2013
  • pp: 25655–25663

High-resolution microscopy for biological specimens via cathodoluminescence of Eu- and Zn-doped Y2O3nanophosphors

Taichi Furukawa, Hirohiko Niioka, Masayoshi Ichimiya, Tomohiro Nagata, Masaaki Ashida, Tsutomu Araki, and Mamoru Hashimoto  »View Author Affiliations

Optics Express, Vol. 21, Issue 22, pp. 25655-25663 (2013)

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High-resolution microscopy for biological specimens was performed using cathodoluminescence (CL) of Y2O3:Eu, Zn nanophosphors, which have high CL intensity due to the incorporation of Zn. The intensity of Y2O3:Eu nanophosphors at low acceleration voltage (3 kV) was increased by adding Zn. The CL intensity was high enough for imaging even with a phosphor size as small as about 30 nm. The results show the possibility of using CL microscopy for biological specimens at single-protein-scale resolution. CL imaging of HeLa cells containing laser-ablated Y2O3:Eu, Zn nanophosphors achieved a spatial resolution of a few tens of nanometers. Y2O3:Eu, Zn nanophosphors in HeLa cells were also imaged with 254 nm ultraviolet light excitation. The results suggest that correlative microscopy using CL, secondary electrons and fluorescence imaging could enable multi-scale investigation of molecular localization from the nanoscale to the microscale.

© 2013 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(180.5810) Microscopy : Scanning microscopy

ToC Category:

Original Manuscript: June 21, 2013
Revised Manuscript: August 6, 2013
Manuscript Accepted: September 9, 2013
Published: October 21, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Taichi Furukawa, Hirohiko Niioka, Masayoshi Ichimiya, Tomohiro Nagata, Masaaki Ashida, Tsutomu Araki, and Mamoru Hashimoto, "High-resolution microscopy for biological specimens via cathodoluminescence of Eu- and Zn-doped Y2O3nanophosphors," Opt. Express 21, 25655-25663 (2013)

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