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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 22 — Nov. 4, 2013
  • pp: 25912–25925

Helical optical projection tomography

Alicia Arranz, Di Dong, Shouping Zhu, Markus Rudin, Christos Tsatsanis, Jie Tian, and Jorge Ripoll  »View Author Affiliations

Optics Express, Vol. 21, Issue 22, pp. 25912-25925 (2013)

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A new technique termed Helical Optical Projection Tomography (hOPT) has been developed with the aim to overcome some of the limitations of current 3D optical imaging techniques. hOPT is based on Optical Projection Tomography (OPT) with the major difference that there is a translation of the sample in the vertical direction during the image acquisition process, requiring a new approach to image reconstruction. Contrary to OPT, hOPT makes possible to obtain 3D-optical images of intact long samples without imposing limits on the sample length. This has been tested using hOPT to image long murine tissue samples such as spinal cords and large intestines. Moreover, 3D-reconstructed images of the colon of DSS-treated mice, a model for Inflammatory Bowel Disease, allowed the identification of the structural alterations. Finally, the geometry of the hOPT device facilitates the addition of a Selective Plane Illumination Microscopy (SPIM) arm, providing the possibility of delivering high resolution images of selected areas together with complete volumetric information.

© 2013 OSA

OCIS Codes
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 10, 2013
Revised Manuscript: September 5, 2013
Manuscript Accepted: September 9, 2013
Published: October 23, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Alicia Arranz, Di Dong, Shouping Zhu, Markus Rudin, Christos Tsatsanis, Jie Tian, and Jorge Ripoll, "Helical optical projection tomography," Opt. Express 21, 25912-25925 (2013)

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