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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 25 — Dec. 16, 2013
  • pp: 30842–30848

In vivo femtosecond endosurgery: an intestinal epithelial regeneration-after-injury model

Myunghwan Choi and Seok Hyun Yun  »View Author Affiliations

Optics Express, Vol. 21, Issue 25, pp. 30842-30848 (2013)

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Regeneration of the intestinal epithelium after injury or during pathogenesis is a dynamic cellular process critical for host immunity. However, current epithelial injury models provide poor spatial control, complicating the study of precise cellular responses. Here we developed endoscopic femtosecond-laser surgery capable of generating acute tissue injury. A side-view probe provides a convenient access to the distal colon in the mouse in vivo and allows real-time intraoperative monitoring as well as pre- and post-surgery examinations via multiphoton imaging. The photo-induced damage showed a nonlinear dependence on laser intensity. At an optical power of 200 mW (2.5 nJ per pulse), scanning the beam focus over 300x300 µm2 area in the colonic mucosa generated substantial vascular damages within 30 s. We confirmed the localized tissue damage and the physiologic regeneration of the disrupted epithelium by in situ barrier function assays, validating the animal model for epithelial regeneration following injury. The femtosecond endosurgery technique is applicable to various experimental models based on laser-induced perturbations.

© 2013 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(170.1020) Medical optics and biotechnology : Ablation of tissue
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 17, 2013
Revised Manuscript: November 3, 2013
Manuscript Accepted: November 12, 2013
Published: December 6, 2013

Virtual Issues
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics

Myunghwan Choi and Seok Hyun Yun, "In vivo femtosecond endosurgery: an intestinal epithelial regeneration-after-injury model," Opt. Express 21, 30842-30848 (2013)

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