OSA's Digital Library

Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 8 — Apr. 22, 2013
  • pp: 10095–10104

Extended depth of field microscopy for rapid volumetric two-photon imaging

Gabrielle Thériault, Yves De Koninck, and Nathalie McCarthy  »View Author Affiliations


Optics Express, Vol. 21, Issue 8, pp. 10095-10104 (2013)
http://dx.doi.org/10.1364/OE.21.010095


View Full Text Article

Enhanced HTML    Acrobat PDF (1642 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Two-photon fluorescence microscopy is an influential tool in biology, providing valuable information on the activity of cells deep inside the tissue. However, it is limited by its low speed for imaging volume samples. Here we present the design of a two-photon scanning microscope with an extended and adjustable depth of field, which improves the temporal resolution for sampling thick samples. Moreover, this method implies no loss of optical power and resolution, and can be easily integrated into most commercial laser-scanning microscopy systems. We demonstrate experimentally the gain in performance of the system by comparing volumetric scans of neuronal structures with a standard versus an extended depth of field system.

© 2013 OSA

OCIS Codes
(180.5810) Microscopy : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: January 11, 2013
Revised Manuscript: March 8, 2013
Manuscript Accepted: March 14, 2013
Published: April 16, 2013

Virtual Issues
Vol. 8, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Gabrielle Thériault, Yves De Koninck, and Nathalie McCarthy, "Extended depth of field microscopy for rapid volumetric two-photon imaging," Opt. Express 21, 10095-10104 (2013)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-8-10095


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods2, 932–40 (2005). [CrossRef] [PubMed]
  2. E. Beaurepaire, M. Oheim, and J. Mertz, “Ultra-deep two-photon fluorescence excitation in turbid media,” Opt. Comm.188, 25–29 (2001). [CrossRef]
  3. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science248, 73–76 (1990). [CrossRef] [PubMed]
  4. W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol.21, 1369–1377 (2003). [CrossRef] [PubMed]
  5. G. D. Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity,” Nat. Neurosci.11, 713–720 (2008). [CrossRef]
  6. W. Göbel, B. M. Kampa, and F. Helmchen, “Imaging cellular network dynamics in three dimensions using fast 3D laser scanning,” Nat. Methods473–79 (2007). [CrossRef]
  7. B. F. Grewe, D. Langer, H. Kasper, B. M. Kampa, and F. Helmchen, “High-speed in vivo calcium imaging reveals neuronal network activity with near-millisecond precision,” Nat. Methods7, 399–405 (2010). [CrossRef] [PubMed]
  8. E. J. Botcherby, C. W. Smith, M. M. Kohl, D. Débarre, M. J. Booth, R. Juškaitis, O. Paulsen, and T. Wilson, “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” PNAS109, 2919–2924 (2012). [CrossRef] [PubMed]
  9. J. H. McLeod, “The axicon: a new type of optical element,” J. Opt. Soc. Am.44, 592–597 (1953). [CrossRef]
  10. N. Olivier, A. Mermillod-Blondin, C. B. Arnold, and E. Beaurepaire, “Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens,” Opt. Lett.34, 1684–1686 (2009). [CrossRef] [PubMed]
  11. E. J. Botcherby, R. Juškaitis, and T. Wilson, “Scanning two photon fluorescence microscopy with extended depth of field.” Opt. Commun.268, 253–260 (2006). [CrossRef]
  12. P. Dufour, M. Piché, Y. De Koninck, and N. McCarthy, “Two-photon excitation fluorescence microscopy with a high depth of field using an axicon,” Appl. Opt.45, 9246–9252 (2006). [CrossRef] [PubMed]
  13. F. O. Fahrbach, P. Simon, and A. Rohrbach, “Microscopy with self-reconstructing beams,” Nature Photon.4, 780–785 (2010). [CrossRef]
  14. T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nature Meth.8, 417–423 (2011). [CrossRef]
  15. A. T. Friberg, “Stationary-phase analysis of generalized axicons,” J. Opt. Soc. Am. A, 13, 743–750 (1996). [CrossRef]
  16. T. A. Pologruto, B. L. Sabatini, and K. Svoboda, “ScanImage: flexible software for operating laser scanning microscopes,” Biomed. Eng. Online2, 13 (2003). [CrossRef] [PubMed]
  17. O. Brzobohatý, T. Čižmár, and P. Zemánek, “High quality quasi-Bessel beam generated by round-tip axicon,” Opt. Express16, 12688–12700 (2008). [CrossRef] [PubMed]
  18. H. Lütcke and F. Helmchen, “Two-photon imaging and analysis of neural network dynamics,” Rep. Prog. Phys.74, 086602 (2011). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4 Fig. 5
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited