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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 21, Iss. 8 — Apr. 22, 2013
  • pp: 10121–10132

Compact surface plasmon-enhanced fluorescence biochip

Koji Toma, Milan Vala, Pavel Adam, Jiří Homola, Wolfgang Knoll, and Jakub Dostálek  »View Author Affiliations

Optics Express, Vol. 21, Issue 8, pp. 10121-10132 (2013)

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A new concept of compact biochip for surface plasmon-enhanced fluorescence assays is reported. It takes advantage of the amplification of fluorescence signal through the coupling of fluorophore labels with confined and strongly enhanced field intensity of surface plasmons. In order to efficiently excite and collect the emitted fluorescence light via surface plasmons on a metallic sensor surface, (reverse) Kretschmann configuration is combined with diffractive optical elements embedded on the chip surface. These include a concentric relief grating for the imaging of highly directional surface plasmon-coupled emission to a detector. Additional linear grating is used for the generating of surface plasmons at the excitation wavelength on the sensor surface in order to increase the fluorescence excitation rate. The reported approach offers the increased intensity of fluorescence signal, reduced background, and compatibility with nanoimprint lithography for cost-effective preparation of sensor chip. The presented approach was implemented for biosensing in a model immunoassay experiment in which the limit of detection of 11 pM was achieved.

© 2013 OSA

OCIS Codes
(050.1950) Diffraction and gratings : Diffraction gratings
(240.6680) Optics at surfaces : Surface plasmons
(300.2530) Spectroscopy : Fluorescence, laser-induced
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(050.6624) Diffraction and gratings : Subwavelength structures

ToC Category:

Original Manuscript: November 27, 2012
Revised Manuscript: January 30, 2013
Manuscript Accepted: February 4, 2013
Published: April 16, 2013

Virtual Issues
Vol. 8, Iss. 5 Virtual Journal for Biomedical Optics

Koji Toma, Milan Vala, Pavel Adam, Jiří Homola, Wolfgang Knoll, and Jakub Dostálek, "Compact surface plasmon-enhanced fluorescence biochip," Opt. Express 21, 10121-10132 (2013)

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