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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 13 — Jun. 30, 2014
  • pp: 16456–16461

Two-photon fluorescence imaging with 30 fs laser system tunable around 1 micron

Bojan Resan, Rodrigo Aviles-Espinosa, Sarah Kurmulis, Jacob Licea-Rodriguez, Felix Brunner, Andreas Rohrbacher, David Artigas, Pablo Loza-Alvarez, and Kurt J. Weingarten  »View Author Affiliations

Optics Express, Vol. 22, Issue 13, pp. 16456-16461 (2014)

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We developed a low-cost, low-noise, tunable, high-peak-power, ultrafast laser system based on a SESAM-modelocked, solid-state Yb tungstate laser plus spectral broadening via a microstructured fiber followed by pulse compression. The spectral selection, tuning, and pulse compression are performed with a simple prism compressor. The output pulses are tunable from 800 to 1250 nm, with the pulse duration down to 25 fs, and average output power up to 150 mW, at 80 MHz pulse repetition rate. We introduce the figure of merit (FOM) for the two-photon and multi-photon imaging (or other nonlinear processes), which is a useful guideline in discussions and for designing the lasers for an improved microscopy signal. Using a 40 MHz pulse repetition rate laser system, with twice lower FOM, we obtained high signal-to-noise ratio two-photon fluorescence images with or without averaging, of mouse intestine section and zebra fish embryo. The obtained images demonstrate that the developed system is capable of nonlinear (TPE, SHG) imaging in a multimodal operation. The system could be potentially used in a variety of other techniques including, THG, CARS and applications such as nanosurgery.

© 2014 Optical Society of America

OCIS Codes
(320.7090) Ultrafast optics : Ultrafast lasers
(320.7160) Ultrafast optics : Ultrafast technology
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Imaging Systems

Original Manuscript: May 28, 2014
Revised Manuscript: June 18, 2014
Manuscript Accepted: June 19, 2014
Published: June 26, 2014

Virtual Issues
Vol. 9, Iss. 8 Virtual Journal for Biomedical Optics

Bojan Resan, Rodrigo Aviles-Espinosa, Sarah Kurmulis, Jacob Licea-Rodriguez, Felix Brunner, Andreas Rohrbacher, David Artigas, Pablo Loza-Alvarez, and Kurt J. Weingarten, "Two-photon fluorescence imaging with 30 fs laser system tunable around 1 micron," Opt. Express 22, 16456-16461 (2014)

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