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Optics Express

Optics Express

  • Editor: Andrew M. Weiner
  • Vol. 22, Iss. 17 — Aug. 25, 2014
  • pp: 20856–20870

High-resolution fluorescence imaging via pattern-illuminated Fourier ptychography

Siyuan Dong, Pariksheet Nanda, Radhika Shiradkar, Kaikai Guo, and Guoan Zheng  »View Author Affiliations

Optics Express, Vol. 22, Issue 17, pp. 20856-20870 (2014)

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Fluorescence microscopy plays a vital role in modern biological research and clinical diagnosis. Here, we report an imaging approach, termed pattern-illuminated Fourier ptychography (FP), for fluorescence imaging beyond the diffraction limit of the employed optics. This approach iteratively recovers a high-resolution fluorescence image from many pattern-illuminated low-resolution intensity measurements. The recovery process starts with one low-resolution measurement as the initial guess. This initial guess is then sequentially updated by other measurements, both in the spatial and Fourier domains. In the spatial domain, we use the pattern-illuminated low-resolution images as intensity constraints for the sample estimate. In the Fourier domain, we use the incoherent optical-transfer-function of the objective lens as the object support constraint for the solution. The sequential updating process is then repeated until the sample estimate converges, typically for 5-20 times. Different from the conventional structured illumination microscopy, any unknown pattern can be used for sample illumination in the reported framework. In particular, we are able to recover both the high-resolution sample image and the unknown illumination pattern at the same time. As a demonstration, we improved the resolution of a conventional fluorescence microscope beyond the diffraction limit of the employed optics. The reported approach may provide an alternative solution for structure illumination microscopy and find applications in wide-field, high-resolution fluorescence imaging.

© 2014 Optical Society of America

OCIS Codes
(100.3190) Image processing : Inverse problems
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3010) Medical optics and biotechnology : Image reconstruction techniques

ToC Category:
Image Processing

Original Manuscript: May 8, 2014
Revised Manuscript: June 27, 2014
Manuscript Accepted: August 4, 2014
Published: August 21, 2014

Virtual Issues
Vol. 9, Iss. 10 Virtual Journal for Biomedical Optics

Siyuan Dong, Pariksheet Nanda, Radhika Shiradkar, Kaikai Guo, and Guoan Zheng, "High-resolution fluorescence imaging via pattern-illuminated Fourier ptychography," Opt. Express 22, 20856-20870 (2014)

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