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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 3, Iss. 9 — Oct. 26, 1998
  • pp: 325–331

Multiphoton laser scanning microscopy for four-dimensional analysis of Caenorhabditis elegans embryonic development.

William A. Mohler and John G. White  »View Author Affiliations

Optics Express, Vol. 3, Issue 9, pp. 325-331 (1998)

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Multiphoton laser scanning microscopy (MPLSM) enables the production of long timelapse recordings from live fluorescent specimens. 1047- and 900-nm excitation were used to image both a vital fluorescent membrane probe, FM 4-64, and a modified green fluorescent protein (GFP) in live Caenorhabditis elegans embryos. Automated four-dimensional (4D) data collection yielded individual recordings comprising thousands of images, each allowing analysis of all of the cell divisions, contacts, migrations, and fusions that occur during a span of several hours of embryogenesis.

© Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Focus Issue: New trends in biomedical microscopy

Original Manuscript: September 18, 1998
Published: October 26, 1998

William Mohler and John White, "Multiphoton laser scanning microscopy for four-dimensional analysis of Caenorhabditis elegans embryonic development.," Opt. Express 3, 325-331 (1998)

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