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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 7, Iss. 12 — Dec. 4, 2000
  • pp: 436–446

Optimal fluorescence excitation wavelengths for detection of squamous intra-epithelial neoplasia: results from an animal model

Lezlee Coghlan, Urs Utzinger, Rebekah Drezek, Douglas Heintzelman, Andres Zuluaga, Carrie Brookner, Rebecca Richards-Kortum, Irma Gimenez-Conti, and Michele Follen  »View Author Affiliations

Optics Express, Vol. 7, Issue 12, pp. 436-446 (2000)

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Using the hamster cheek pouch carcinogenesis model, we explore which fluorescence excitation wavelengths are useful for the detection of neoplasia. 42 hamsters were treated with DMBA to induce carcinogenesis, and 20 control animals were treated only with mineral oil. Fluorescence excitation emission matrices were measured from the cheek pouches of the hamsters weekly. Results showed increased fluorescence near 350–370 nm and 410 nm excitation and decreased fluorescence near 450–470 nm excitation with neoplasia. The optimal diagnostic excitation wavelengths identified using this model - 350–370 nm excitation and 400–450 nm excitation - are similar to those identified for detection of human oral cavity neoplasia.

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OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Research Papers

Original Manuscript: October 23, 2000
Published: December 4, 2000

Lezlee Coghlan, Urs Utzinger, Rebekah Drezek, Doug Heintzelmann, Andres Zuluaga, Carrie Brookner, Rebecca Richards-Kortum, Irma Gimenez-Conti, and Michele Follen, "Optimal fluorescence excitation wavelengths for detection of squamous intra-epithelial neoplasia: results from an animal model," Opt. Express 7, 436-446 (2000)

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